Mallet C, Mallet V N
Chemistry and Biochemistry Department, Université de Moncton, Canada.
J Chromatogr. 1989 Nov 3;481:27-35. doi: 10.1016/s0021-9673(01)96749-4.
A conventional packed-column chromatograph was modified for use with a megabore column using a commercial conversion kit, with the intention of developing a multi-residue method for organophosphorus pesticides. The results indicate that the conversion does not affect the resolving power of the megabore column, since fourteen organophosphorus pesticides could be separated in a single injection. The megabore column in such a system proved to be much more efficient than a packed column in terms of resolution and qualitative reproducibility, and comparable with a similar column in a dedicated chromatograph. However, the conversion did not affect the quantitative reproducibility of the system to some degree, as indicated by coefficients of variation between 5 and 27%, although it was determined that the particular nitrogen-phosphorus detector system (filament bead) used in this study was partly responsible for the variation. Nevertheless, calibration curves were obtained down to 0.1 ng per component, and a limit of quantitation of 1.0 ng was established for each component in a sample containing fourteen organophosphorus compounds. The results indicate that the system is sufficiently reproducible to develop a multi-residue method for organophosphorus pesticides in environmental waters.
使用商用转换套件对传统填充柱色谱仪进行改装，使其适用于大口径柱，目的是开发一种有机磷农药多残留分析方法。结果表明，这种转换不会影响大口径柱的分离能力，因为一次进样就能分离出14种有机磷农药。在该系统中，大口径柱在分离度和定性重现性方面比填充柱高效得多，与专用色谱仪中的类似柱相当。然而，转换在一定程度上并未影响系统的定量重现性，变异系数在5%至27%之间，不过已确定本研究中使用的特定氮磷检测系统（细丝珠）是造成变异的部分原因。尽管如此，仍获得了各组分低至0.1 ng的校准曲线，并为含有14种有机磷化合物的样品中的每种组分确定了1.0 ng的定量限。结果表明，该系统具有足够的重现性，可用于开发环境水中有机磷农药的多残留分析方法。
