Highly sensitive and specific non-radioactive biotinylated probes for dot-blot, Southern and colony hybridizations.
作者信息
Eweida M, Sit T L, Sira S, AbouHaidar M G
机构信息
Department of Botany, University of Toronto, Ontario, Canada.
出版信息
J Virol Methods. 1989 Oct;26(1):35-43. doi: 10.1016/0166-0934(89)90072-4.
PMID:2592520
Abstract
We describe a simple method for the incorporation of biotin into nucleic acid probes. This method has been improved and optimized to produce biotinylated DNA probes for the detection of DNA by dot-blot, Southern and colony hybridization techniques. The sensitivity of this method has been particularly improved to allow detection of DNA quantities under one femtogram. Probes prepared by this method are highly specific for target DNA even in crude bacterial lysates.
摘要
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