Liu Tingting, Huang Canke, Shen Chaofeng, Shi Jiyan
Institute of Environmental Science and Technology, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, 310058, People's Republic of China.
Appl Biochem Biotechnol. 2015 Jun;176(4):1174-94. doi: 10.1007/s12010-015-1638-7. Epub 2015 May 1.
Cell wall proteins (CWPs) are a prime site for signal perception and defense responses to environmental stresses. To gain further insights into CWPs and their molecular function, traditional techniques (e.g., two-dimensional gel electrophoresis) may be ineffective for special proteins. Elsholtzia splendens is a copper-tolerant plant species that grow on copper deposits. In this study, a fourplex isobaric tag was used for relative and absolute quantitation with liquid chromatography-tandem mass spectrometry approach to analyze the root CWPs of E. splendens. A total of 479 unique proteins were identified, including 121 novel proteins. Approximately 80.79 % of the proteins were extracted in the CaCl2 fraction, 16.08 % were detected in the NaCl fraction, and 3.13 % were identified in both fractions. The identified proteins have been involved in various processes, including cell wall remodeling, signal transduction, defense, and carbohydrate metabolism, thereby indicating a complex regulatory network in the apoplast of E. splendens roots. This study presents the first large-scale analysis of CWPs in metal-tolerant plants, which may be of paramount importance to understand the molecular functions and metabolic pathways in the root cell wall of copper-tolerant plants.
细胞壁蛋白（CWPs）是植物感知信号和对环境胁迫产生防御反应的主要位点。为了更深入了解CWPs及其分子功能，传统技术（如二维凝胶电泳）可能对某些特殊蛋白质无效。海州香薷是一种生长在铜矿上的耐铜植物。在本研究中，采用四重等压标记结合液相色谱-串联质谱法对海州香薷根系的CWPs进行相对和绝对定量分析。共鉴定出479种独特蛋白质，其中包括121种新蛋白质。约80.79%的蛋白质在CaCl2组分中被提取，16.08%在NaCl组分中被检测到，3.13%在两个组分中均被鉴定出。所鉴定的蛋白质参与了包括细胞壁重塑、信号转导、防御和碳水化合物代谢等多种过程，这表明海州香薷根质外体中存在一个复杂的调控网络。本研究首次对耐金属植物中的CWPs进行了大规模分析，这对于理解耐铜植物根细胞壁的分子功能和代谢途径可能具有至关重要的意义。
