Burton J L, Kennedy B W, Burnside E B, Wilkie B N, Burton J H
Centre for Genetic Improvement of Livestock, University of Guelph, Ontario, Canada.
J Dairy Sci. 1989 Sep;72(9):2351-61. doi: 10.3168/jds.S0022-0302(89)79368-1.
Measurements of double skin fold thickness (mm) were used to assess the feasibility of use of cutaneous delayed-type hypersensitivity response as a marker trait to select for genetically improved disease resistance. Data were from a sample of 149 7-wk-old Holstein calves, from 15 sires, that previously had been sensitized to the contact allergen dinitrochlorobenzene. Response was monitored 24 and 48 h postchallenge. Analysis was by least squares according to a model that included fixed managerial effects, disease prevalence, and severity categories and sire of calf. Double skin fold thickness increased about 50% by 24 h, followed by a 4% decrease between 24 and 48 h. Season of birth was the only consistently significant fixed effect. Calves born in fall showed larger skin changes at each stage of response than calves born in spring. Restricted maximum likelihood estimates of paternal half-sib heritability were moderate to high for all stages of response, and phenotypic and genetic correlations between stages were significant and positive. Although the prevalence and severity of naturally occurring pneumonia and diarrhea did not significantly affect quantity of response, there is need to study further this relationship for intracellular pathogens. Delayed-type hypersensitivity response to dinitrochlorobenzene can meet requirements for a successful marker on which to base sire selection for disease resistance, if it is related to economically important, intracellularly characterized, pathogenic bovine diseases.
采用双皮褶厚度（毫米）测量法来评估将皮肤迟发型超敏反应作为一种标记性状用于选择遗传改良抗病性的可行性。数据来自149头7周龄荷斯坦犊牛的样本，这些犊牛来自15头公牛，此前已对接触性过敏原二硝基氯苯致敏。在激发后24小时和48小时监测反应。分析采用最小二乘法，依据一个包含固定管理效应、疾病患病率、严重程度类别以及犊牛父亲的模型进行。双皮褶厚度在24小时时增加了约50%，随后在24小时至48小时之间下降了4%。出生季节是唯一始终具有显著影响的固定效应。秋季出生的犊牛在反应的各个阶段都比春季出生的犊牛表现出更大的皮肤变化。父本半同胞遗传力的限制最大似然估计在反应的所有阶段均为中度到高度，且各阶段之间的表型和遗传相关性显著且为正。尽管自然发生的肺炎和腹泻的患病率及严重程度对反应量没有显著影响，但对于细胞内病原体，仍需进一步研究这种关系。如果对二硝基氯苯的迟发型超敏反应与经济上重要的、具有细胞内特征的致病性牛病相关，那么它可以满足作为抗病性父本选择成功标记的要求。
