Maebe Kevin, Meeus Ivan, De Riek Jan, Smagghe Guy
Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure links 653, B-9000, Ghent, Belgium.
Institute for Agricultural and Fisheries Research (ILVO), Plant Sciences Unit, Caritasstraat 21, B-9090, Melle, Belgium.
PLoS One. 2015 Apr 30;10(4):e0125011. doi: 10.1371/journal.pone.0125011. eCollection 2015.
Bumblebees such as Bombus terrestris are essential pollinators in natural and managed ecosystems. In addition, this species is intensively used in agriculture for its pollination services, for instance in tomato and pepper greenhouses. Here we performed a quantitative trait loci (QTL) analysis on B. terrestris using 136 microsatellite DNA markers to identify genes linked with 20 traits including light sensitivity, body size and mass, and eye and hind leg measures. By composite interval mapping (IM), we found 83 and 34 suggestive QTLs for 19 of the 20 traits at the linkage group wide significance levels of p = 0.05 and 0.01, respectively. Furthermore, we also found five significant QTLs at the genome wide significant level of p = 0.05. Individual QTLs accounted for 7.5-53.3% of the phenotypic variation. For 15 traits, at least one QTL was confirmed with multiple QTL model mapping. Multivariate principal components analysis confirmed 11 univariate suggestive QTLs but revealed three suggestive QTLs not identified by the individual traits. We also identified several candidate genes linked with light sensitivity, in particular the Phosrestin-1-like gene is a primary candidate for its phototransduction function. In conclusion, we believe that the suggestive and significant QTLs, and markers identified here, can be of use in marker-assisted breeding to improve selection towards light sensitive bumblebees, and thus also the pollination service of bumblebees.
诸如地熊蜂之类的大黄蜂是自然和人工管理生态系统中至关重要的传粉者。此外，由于其授粉服务，该物种在农业中被广泛应用，例如在番茄和辣椒温室中。在此，我们使用136个微卫星DNA标记对地熊蜂进行了数量性状基因座（QTL）分析，以鉴定与20个性状相关的基因，这些性状包括光敏感性、体型和体重，以及眼睛和后腿的测量数据。通过复合区间作图（IM），我们分别在连锁群全基因组显著水平p = 0.05和0.01时，为20个性状中的19个找到了83个和34个暗示性QTL。此外，我们还在全基因组显著水平p = 0.05时发现了5个显著QTL。单个QTL占表型变异的7.5 - 53.3%。对于15个性状，至少有一个QTL通过多QTL模型作图得到了确认。多变量主成分分析确认了11个单变量暗示性QTL，但揭示了3个未通过单个性状鉴定出的暗示性QTL。我们还鉴定了几个与光敏感性相关的候选基因，特别是类磷酸视蛋白-1基因因其光转导功能而成为主要候选基因。总之，我们认为这里鉴定出的暗示性和显著QTL以及标记，可用于标记辅助育种，以改进对光敏感大黄蜂的选择，从而也改善大黄蜂的授粉服务。
