Bukowski R, Wąsowicz K
Pol J Vet Sci. 2015;18(1):199-206. doi: 10.1515/pjvs-2015-0025.
The presence and distribution of vasoactive intestinal polypeptide (VIP) receptor VPAC1 was studied in the ovary, oviduct and uterus (uterine horn and cervix) of the domestic pig using methods of molecular biology (RT-PCR and immunoblot) and immunohistochemistry. The expression of VPAC1 receptor at mRNA level was confirmed with RT-PCR in all the studied parts of the porcine female reproductive system by the presence of 525 bp PCR product and at the level of proteins by the detection of 46 kDa protein band in immunoblot. Immunohistochemical stainings revealed the cellular distribution of VPAC1 receptor protein. In the ovary it was present in the wall of arterial blood vessels, as well as in the ovarian follicles of different stages. In the tubular organs the VPAC1 receptor immunohistochemical stainings were observed in the wall of the arterial blood vessels, in the muscular membrane, as well as in the mucosal epithelium. The study confirmed the presence of VPAC1 receptor in the tissues of the porcine female reproductive tract what clearly shows the possibility of influence of VIP on the porcine ovary, oviduct and uterus.
采用分子生物学方法（逆转录聚合酶链反应和免疫印迹法）及免疫组织化学方法，研究了血管活性肠肽（VIP）受体VPAC1在家猪卵巢、输卵管和子宫（子宫角和子宫颈）中的存在情况及分布。通过逆转录聚合酶链反应，在猪雌性生殖系统所有被研究部位均检测到525 bp的聚合酶链反应产物，从而证实了VPAC1受体在mRNA水平的表达；通过免疫印迹法检测到46 kDa的蛋白条带，证实了其在蛋白质水平的表达。免疫组织化学染色揭示了VPAC1受体蛋白的细胞分布。在卵巢中，它存在于动脉血管壁以及不同阶段的卵泡中。在管状器官中，VPAC1受体免疫组织化学染色见于动脉血管壁、肌膜以及黏膜上皮。该研究证实了VPAC1受体存在于猪雌性生殖道组织中，这清楚地表明了血管活性肠肽对猪卵巢、输卵管和子宫产生影响的可能性。
