荷斯坦-弗里生（HF）人工授精公牛冷冻精液的选定质量和生化参数。

Selected qualitative and biochemical parameters of cryopreserved semen of Holstein-Friesian (HF) AI bulls.

作者信息

Lecewicz M, Hering D M, Kamiński S, Majewska A, Kordan W

出版信息

Pol J Vet Sci. 2015;18(1):237-9. doi: 10.1515/pjvs-2015-0030.

DOI:10.1515/pjvs-2015-0030

Abstract

Selected qualitative and biochemical parameters were determined in cryopreserved semen used for artificial insemination, sampled from 120 bulls reared at the Animal Breeding and Insemination Center in Bydgoszcz. The total average motility of the analyzed sperm samples was determined at 62.51%. The percentage of motile spermatozoa displaying progressive forward motility was 21.65%. Analyzed samples were characterized by a high percentage of sperm cells with a intact plasma membrane (71.21%) and active mitochondria (71.32%). High efficiency of the enzymatic antioxidant system of the evaluated sperm cells was demonstrated by high activity of CAT, GPx and SOD (494.37, 2847.83 and 5.31U/1x10(9) spermatozoa, respectively) values and low values of the DNA Fragmentation Index (9.32). The results of the study, obtained with the involvement of advanced analytical methods, indicate a high fertilizing capability of the analyzed sperm samples.

摘要

对从比得哥什动物育种与授精中心饲养的120头公牛采集的用于人工授精的冷冻精液，测定了选定的定性和生化参数。所分析精子样本的总平均活力测定为62.51%。表现出渐进性向前运动的活动精子百分比为21.65%。所分析的样本的特征是具有完整质膜的精子细胞百分比高（71.21%）和活跃线粒体百分比高（71.32%）。评估的精子细胞的酶促抗氧化系统的高效率通过CAT、GPx和SOD的高活性（分别为494.37、2847.83和5.31U/1x10(9)精子）值和低DNA碎片化指数值（9.32）得到证明。通过先进分析方法获得的研究结果表明，所分析的精子样本具有很高的受精能力。