聚山梨酯80包被的白藜芦醇负载聚丙交酯纳米颗粒对MPTP诱导的帕金森病模型神经保护作用的增强
Improved neuroprotective effects of resveratrol-loaded polysorbate 80-coated poly(lactide) nanoparticles in MPTP-induced Parkinsonism.
作者信息
da Rocha Lindner Gabriela, Bonfanti Santos Danúbia, Colle Dirleise, Gasnhar Moreira Eduardo Luiz, Daniel Prediger Rui, Farina Marcelo, Khalil Najeh Maissar, Mara Mainardes Rubiana
机构信息
Department of Pharmacy, Laboratory of Pharmaceutical Nanotechnology, Universidade Estadual do Centro-Oeste/UNICENTRO, Rua Simeão Camargo Varela de Sá, 03, 85040-080, Guarapuava, PR, Brazil.
出版信息
Nanomedicine (Lond). 2015;10(7):1127-38. doi: 10.2217/nnm.14.165.
AIM
This study investigated the neuroprotective effects of resveratrol (RVT)-loaded polysorbate 80 (PS80)-coated poly(lactide) nanoparticles in a mouse model of Parkinson's disease (PD), and compared these effects with those from bulk RVT.
METHODS
C57BL/6 mice received for 15 days RVT intraperitoneally (nanoparticulate or non-nanoparticulate), as well as single intranasal administration of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), a neurotoxin that damages dopaminergic neurons and induces PD-related symptoms.
RESULTS
MPTP induced significant impairments on olfactory discrimination and social recognition memory, as well as caused striatal oxidative stress and reduced the expression of tyrosine hydroxylase in striatum. RVT-loaded nanoparticles (but not bulk) displayed significant neuroprotection against MPTP-induced behavioral and neurochemical changes.
CONCLUSION
These results point to RVT-loaded poly(lactide)-nanoparticles coated with PS80 a promising nanomedical tool and adjuvant therapy for PD.
目的
本研究在帕金森病（PD）小鼠模型中研究了载有白藜芦醇（RVT）的聚山梨酯80（PS80）包被的聚丙交酯纳米颗粒的神经保护作用，并将这些作用与游离RVT的作用进行比较。
方法
C57BL/6小鼠连续15天腹腔注射RVT（纳米颗粒形式或非纳米颗粒形式），并单次鼻内给予1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP），一种损害多巴胺能神经元并诱发PD相关症状的神经毒素。
结果
MPTP对嗅觉辨别和社会识别记忆造成显著损害，导致纹状体氧化应激，并降低纹状体中酪氨酸羟化酶的表达。载有RVT的纳米颗粒（而非游离RVT）对MPTP诱导的行为和神经化学变化显示出显著的神经保护作用。
结论
这些结果表明，载有RVT的PS80包被的聚丙交酯纳米颗粒是一种有前景的纳米医学工具和PD辅助治疗手段。
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