Taki Masumi, Kuroiwa Hiroyuki
Division of Chemistry and Biochemistry, Department of Medical and Bioengineering Science, Chemical Biology Area, Okayama University, 1-1-1, Tsushimanaka, Okayama, 700-0082, Japan,
Amino Acids. 2015 Jun;47(6):1279-82. doi: 10.1007/s00726-015-1989-y. Epub 2015 May 1.
This article demonstrates the fastest enzymatic introduction of a positron emission tomography (PET) probe into acceptor peptides/proteins. It is site-specifically introduced at the basic N-terminus of the acceptors by using L/F-transferase in combination with aminoacyl-tRNA synthetase, namely the NEXT-A/PET reaction. Estimated from kinetic analysis, the transfer efficiency of O-(2-fluoromethyl)-L-tyrosine as an artificial amino acid PET probe mediated by the wild-type transferase is almost as good as that of the natural substrate, phenylalanine.
本文展示了将正电子发射断层扫描（PET）探针最快地酶促引入受体肽/蛋白质的方法。通过使用L/F-转移酶与氨酰-tRNA合成酶相结合，即NEXT-A/PET反应，将其位点特异性地引入受体的碱性N端。根据动力学分析估计，由野生型转移酶介导的作为人工氨基酸PET探针的O-（2-氟甲基）-L-酪氨酸的转移效率几乎与天然底物苯丙氨酸的转移效率一样好。
