Wu Xu Na, Schulze Waltraud X
Department of Plant Systems Biology, Universität Hohenheim, Fruwirthstraße 12, Stuttgart, 70593, Germany.
Methods Mol Biol. 2015;1306:71-9. doi: 10.1007/978-1-4939-2648-0_5.
The family of transmembrane receptor kinase is the largest protein kinase family in Arabidopsis. However many of these kinases have yet uncharacterized functions and little is known about direct substrates of these kinases. Here, we present a large-scale phosphoproteomics method involving label-free quantitation-based comparative phosphopeptide profiling of knockout mutants in receptor-like kinases. This approach, among other physiological and cell biological experiments, is one step in understanding the functional roles of plant kinases in the context of their signaling networks.
跨膜受体激酶家族是拟南芥中最大的蛋白激酶家族。然而，这些激酶中的许多功能尚未明确，对其直接底物也知之甚少。在此，我们提出了一种大规模磷酸化蛋白质组学方法，该方法涉及基于无标记定量的受体样激酶基因敲除突变体的磷酸化肽段比较分析。除其他生理和细胞生物学实验外，这种方法是在植物激酶信号网络背景下理解其功能作用的其中一步。
