Zhang Yingxia, Wang Tiansheng, Cao Yu
Department of Orthopedics, 463 Hospital of PLA Shenyang 110042, P.R. China.
Department of Physiology, China Medical University Shenyang 110042, P.R. China.
Int J Clin Exp Med. 2015 Feb 15;8(2):2254-60. eCollection 2015.
To observe the effect of osteopontin (OPN) on the expression of collagen type II (Col-II) and oligomeric matrix protein COMP in cartilage cells of knee osteoarthritis, and explore the mechanism of OPN in the knee osteoarthritis.
Cartilage cells were isolated from fetal rabbit, they were divided into 3 groups: A (control); B (0.5 μM OPN) and C (1 μM OPN). The expression levels of Col-2 and COMP were detected by RT-PCR and Western blotting methods. MMP-13 was detected using ELISA. The proliferation was determined by MTT method.
Compared with the group, the expression levels of Col-2 and COMP in cartilage cells decreased in the intervention group with dose dependent (P<0.05). The expression of MMP-13 in culture supernatant and the proliferation increased in the intervention group (P<0.05).
OPN can down-regulate the expression levels of Col-2 and COMP in cartilage cells and up-regulate the expression of MMP-13 in culture supernatant and promote the proliferation of cells, which could accelerate the pathological process of cartilage cells.
观察骨桥蛋白（OPN）对膝骨关节炎软骨细胞中Ⅱ型胶原蛋白（Col-II）和寡聚基质蛋白COMP表达的影响，探讨OPN在膝骨关节炎中的作用机制。
从胎兔分离软骨细胞，分为3组：A组（对照组）；B组（0.5 μM OPN）和C组（1 μM OPN）。采用RT-PCR和蛋白质印迹法检测Col-2和COMP的表达水平。用ELISA法检测MMP-13。采用MTT法测定细胞增殖。
与对照组相比，干预组软骨细胞中Col-2和COMP的表达水平呈剂量依赖性降低（P<0.05）。干预组培养上清中MMP-13的表达及细胞增殖增加（P<0.05）。
OPN可下调软骨细胞中Col-2和COMP的表达水平，上调培养上清中MMP-13的表达并促进细胞增殖，从而加速软骨细胞的病理进程。
