Biosurface Technology Division, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala 695012, India.
Biosurface Technology Division, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala 695012, India.
Carbohydr Polym. 2015 Aug 1;126:52-61. doi: 10.1016/j.carbpol.2015.03.023. Epub 2015 Mar 21.
Cationized pullulan (pullulan-PEI; PP) was synthesized and further modified with an anti-oxidant molecule, ascorbic acid (PPAA) at various ratios. The nanoplexes formed at an optimum ratio of 4:1 was within a size of 150nm and had a zeta potential of 9-14mV. The nanoplexes at this ratio was used for further investigations. The cell internalization and transfection efficiency of these nanoplexes were determined in presence of serum. The internalization and transfection efficiency were found to be unaffected by the presence of fetal bovine serum. Another interesting observation was that this polymer was found to have collagen synthesis promoting property. The collagen synthesis effect of these polymers was quantified and observed that PPAA3 promoted the highest. Transfection efficiency was evaluated by assessing the p53 gene expression in C6 rat glioma cells and cell death was quantified to be 96% by flow cytometry, thus establishing the high efficacy of this polymer.
阳离子化支链淀粉（支链淀粉-PEI；PP）被合成，并与抗氧化剂分子抗坏血酸（PPAA）以不同比例进一步修饰。在最佳比例 4:1 下形成的纳米复合物的粒径为 150nm，zeta 电位为 9-14mV。在此比例下使用纳米复合物进行进一步研究。在存在血清的情况下，确定了这些纳米复合物的细胞内化和转染效率。发现细胞内化和转染效率不受胎牛血清的影响。另一个有趣的观察结果是，这种聚合物被发现具有促进胶原蛋白合成的特性。对这些聚合物的胶原蛋白合成效果进行定量，并观察到 PPAA3 促进作用最高。通过评估 C6 大鼠神经胶质瘤细胞中 p53 基因的表达来评估转染效率，并通过流式细胞术定量细胞死亡为 96%，从而证实了这种聚合物的高效性。
