College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 210043, China.
College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 210043, China.
Carbohydr Polym. 2015 Aug 1;126:179-84. doi: 10.1016/j.carbpol.2015.03.011. Epub 2015 Mar 17.
Broccoli is a widely consumed vegetable with abundant amount of nutrients, which bring numerous beneficial effects on human health. The structural information of water-soluble polysaccharides in broccoli was eludicated for the first time in this work. A purified polysaccharide fraction (BPCa) was obtained by column chromatography. It comprised of arabinose (Ara), galactose (Gal), rhamnose (Rha) with a molar ratio of 5.3:0.8:1.0. Nuclear magnetic resonnance spectra data revealed that α-L-1,5-Araf and α-L-1,3,5-Araf are present in the backbone, while α-L-Araf terminal was attended in side chain. α-L-1,2-Rhap was found to be linked to α-L-1,5-Araf in heteronuclear multiple bond correlation spectra. The presences of β-D-1,4-Galp and α-D-1,4-GalpA were also detected. Furthermore, BPCa showed significant anti-cancer cell proliferation activities against HepG2, Siha and MDA-MB-231 carcinoma cell lines. The results indicated that BPCa had a good potential to be applied as functional food additives.
西兰花是一种广泛食用的蔬菜，含有丰富的营养成分，对人体健康有许多有益的影响。本工作首次阐明了西兰花水溶性多糖的结构信息。通过柱层析得到了一种纯化的多糖级分（BPCa）。它由阿拉伯糖（Ara）、半乳糖（Gal）、鼠李糖（Rha）组成，摩尔比为 5.3:0.8:1.0。核磁共振波谱数据表明，α-L-1,5-Araf 和 α-L-1,3,5-Araf 存在于主链中，而α-L-Araf 末端存在于侧链中。在异核多键相关光谱中发现α-L-1,2-Rhap 与α-L-1,5-Araf 相连。还检测到β-D-1,4-Galp 和α-D-1,4-GalpA 的存在。此外，BPCa 对 HepG2、Siha 和 MDA-MB-231 癌细胞系表现出显著的抑制癌细胞增殖活性。结果表明，BPCa 具有作为功能性食品添加剂的良好应用潜力。
