簇集素转录变体在甲状腺肿瘤中的表达：一种潜在的恶性肿瘤标志物？
Clusterin transcript variants expression in thyroid tumor: a potential marker of malignancy?
作者信息
Fuzio Paolo, Napoli Anna, Ciampolillo Anna, Lattarulo Serafina, Pezzolla Angela, Nuzziello Nicoletta, Liuni Sabino, Giorgino Francesco, Maiorano Eugenio, Perlino Elda
机构信息
Institute of Biomedical Technologies, National Research Council (CNR), Via G. Amendola, 122/D, 70126, Bari, Italy.
Department of Emergency and Organ Transplantation, Section of Pathological Anatomy, University of Bari Aldo Moro, 70124, Bari, Italy.
出版信息
BMC Cancer. 2015 May 2;15:349. doi: 10.1186/s12885-015-1348-0.
BACKGROUND
Clusterin (CLU) is a ubiquitous multifunctional factor involved in neoplastic transformation. The CLU transcript variants and protein forms play a crucial role in balancing cells proliferation and death.
METHODS
We investigated the regulation of CLU transcript variants expression in an in vivo model system consisting of both neoplastic tissues and fine needle aspiration biopsy (FNAB) samples isolated from patients undergoing thyroidectomy.
RESULTS
The immunohistochemical analyses showed an overall CLU up-regulation in papillary carcinoma. A specific CLU2 transcript variant increase was registered using qPCR in papillary carcinomas while CLU1 decreased. In addition, the analysis of CLU transcripts expression level showed an increase of the CLU2 transcript in the TIR 3 patients with histologically confirmed thyroid cancer.
CONCLUSIONS
Our results suggest the existence of a specific alteration of CLU2:CLU1 ratio towards CLU2, thus providing the first circumstantial evidence for the potential use of CLU transcript variants as effective biomarkers for a more accurate assessment of the so called "indeterminate" thyroid nodules.
背景
簇集素（CLU）是一种广泛存在的多功能因子，参与肿瘤转化过程。CLU转录变体和蛋白质形式在平衡细胞增殖和死亡中起关键作用。
方法
我们在一个体内模型系统中研究了CLU转录变体表达的调控，该系统由肿瘤组织和从接受甲状腺切除术的患者中分离的细针穿刺活检（FNAB）样本组成。
结果
免疫组织化学分析显示，乳头状癌中CLU总体上调。使用qPCR检测发现，乳头状癌中特定的CLU2转录变体增加，而CLU1减少。此外，对CLU转录本表达水平的分析表明，在组织学确诊为甲状腺癌的TIR 3患者中，CLU2转录本增加。
结论
我们的结果表明存在CLU2:CLU1比例向CLU2的特定改变，从而为将CLU转录变体作为有效的生物标志物用于更准确评估所谓的“不确定”甲状腺结节提供了首个间接证据。
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