Bautista-Garfias Carlos Ramón, Lozano Astrid Rodríguez, Martínez Carmen Rojas, Martínez Jesús Antonio Álvarez, Millán Julio Vicente Figueroa, García Gustavo Román Reyes, Castañeda-Arriola Roberto, Aguilar-Figueroa Blanca Rosa
Unidad de Babesia, Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria (CENID-PAVET), Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), Mexico.
Unidad de Babesia, Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria (CENID-PAVET), Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), Mexico.
Parasitol Int. 2015 Oct;64(5):319-23. doi: 10.1016/j.parint.2015.04.005. Epub 2015 Apr 30.
The effect of Lactobacillus casei administered along with a live attenuated vaccine vs. bovine babesiosis (VAC) on induction of IgG1 and IgG2 antibodies to Babesia bovis and Babesia bigemina was assessed by the indirect fluorescent antibody test (IFAT) in bovines of an endemic babesiosis area before (day 0) and after vaccination (days 15 and 30). We previously reported that L. casei increases the efficiency of VAC under controlled conditions and under extreme conditions in the field; however, the levels of IgG1 and IgG2 antibodies to B. bovis and B. bigemina are not known in vaccinated animals. Twenty-one dairy cows were allocated into three groups (seven animals per group): unvaccinated, vaccinated with VAC and vaccinated simultaneously with VAC and L. casei (VAC-LC). All animals were kept in a babesiosis endemic area at Tlalixcoyan, Veracruz. At days 15 and 30 after vaccination, the average levels of IgG1 to B. bovis and to B. bigemina were significantly higher in VAC-LC group than levels observed in VAC and control groups (P<0.01). Levels of IgG2 were similar in VAC and VAC-LC groups but higher than in the control group (P<0.01). When tested in in vitro cultures of B. bovis, sera from VAC-LC group significantly inhibited parasite growth as compared with the sera of the other two groups. It is suggested that the efficiency improvement of VAC, in part, is due to the L. casei boost of IgG1 over IgG2 antibodies to B. bovis and B. bigemina when the bacteria is co-inoculated with this vaccine.
通过间接荧光抗体试验（IFAT），在一个牛巴贝斯虫病流行地区的牛群中，于接种减毒活疫苗（VAC）预防牛巴贝斯虫病前（第0天）以及接种后第15天和第30天，评估了干酪乳杆菌与VAC联合使用对诱导针对牛巴贝斯虫和双芽巴贝斯虫的IgG1和IgG2抗体的影响。我们之前报道过，在可控条件下以及在田间极端条件下，干酪乳杆菌可提高VAC的效力；然而，接种疫苗动物体内针对牛巴贝斯虫和双芽巴贝斯虫的IgG1和IgG2抗体水平尚不清楚。将21头奶牛分为三组（每组7头）：未接种组、接种VAC组以及同时接种VAC和干酪乳杆菌组（VAC-LC）。所有动物均饲养在韦拉克鲁斯州特拉利斯科扬的一个巴贝斯虫病流行地区。接种后第15天和第30天，VAC-LC组中针对牛巴贝斯虫和双芽巴贝斯虫的IgG1平均水平显著高于VAC组和对照组（P<0.01）。VAC组和VAC-LC组的IgG2水平相似，但高于对照组（P<0.01）。在牛巴贝斯虫的体外培养中进行检测时，与其他两组血清相比，VAC-LC组血清显著抑制了寄生虫生长。提示VAC效力的提高部分归因于当干酪乳杆菌与该疫苗共同接种时，其对针对牛巴贝斯虫和双芽巴贝斯虫的IgG1抗体而非IgG2抗体的增强作用。
