生物发光全细胞报告基因检测作为筛选工具用于鉴定抗菌天然产物提取物。
Bioluminescent whole-cell reporter gene assays as screening tools in the identification of antimicrobial natural product extracts.
作者信息
Nybond Susanna, Karp Matti, Yrjönen Teijo, Tammela Päivi
机构信息
Centre for Drug Research, Division of Pharmaceutical Biosciences, University of Helsinki, Helsinki, Finland.
Department of Chemistry and Bioengineering, Tampere University of Technology, Tampere, Finland.
出版信息
J Microbiol Methods. 2015 Jul;114:54-6. doi: 10.1016/j.mimet.2015.04.014. Epub 2015 Apr 30.
We describe novel tools, bioluminescent whole-cell reporter gene assays, for facilitating the use of natural products in antimicrobial drug discovery. As proof-of-concept, a plant extract library was screened and follow-up experiments were carried out. Primary results can be obtained in 2-4h with high sensitivity, leading to significant improvements of the process.
我们描述了一种新型工具——生物发光全细胞报告基因检测法，以促进天然产物在抗菌药物发现中的应用。作为概念验证，我们筛选了一个植物提取物文库并进行了后续实验。主要结果可在2-4小时内以高灵敏度获得，从而显著改进了该过程。
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