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用于生物染料薄层色谱分析的溶剂系统。

Solvent systems for thin layer chromatography of biological dyes.

作者信息

Allison R T, Garratt N J

出版信息

Med Lab Sci. 1989 Apr;46(2):113-9.

PMID:2593771
Abstract

Thirty-nine dyes were subjected to thin layer chromatography to evaluate eight different solvent systems. The results obtained were analysed by dye class following assessment in daylight and ultra violet light. N-butanol: acetic acid: water (40:10:50) gave optimum separations of dyes viewed in visible light. Although n-butanol: ethanol: water (90:10:10) gave slightly better results for fluorescing compounds, material was frequently left at the start point with this solvent.

摘要

对39种染料进行了薄层色谱分析,以评估8种不同的溶剂系统。在日光和紫外光下进行评估后,按照染料类别对所得结果进行分析。正丁醇:乙酸:水(40:10:50)在可见光下对染料有最佳分离效果。虽然正丁醇:乙醇:水(90:10:10)对荧光化合物的效果略好,但使用这种溶剂时,物质常常留在起始点。

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