Liu Feng, Sun Jian, Li Ya-li, Chen Li-qiang, Xie Jing-sen, Zang Xiao-long
Department of Oral and Maxillofacial Surgery, The Affiliated Hospital of Qingdao University. Qingdao 266003, Shandong Province, China.E-mail:
Shanghai Kou Qiang Yi Xue. 2015 Apr;24(2):147-50.
To prepare chitosan nanospheres for loading of BMP-2 and to evaluate its size, zeta potential, appearance, degradation and release characteristic in vitro, and then to investigate its feasibility as a carrier for sustained release of BMP-2.
The BMP-2 loaded chitosan nanospheres were prepared using ionic crosslinking method with tripolyphosphate (TPP) and chitosan. Transmission electron microscope was used to evaluate the morphological properties, and laser particle size analyzer was used to analyze particle size, Zeta potential and distribution. Lysozyme degradation experiment was performed to assess the biodegradation behavior. ELISA assay was used to determine the loading efficiency, encapsulation efficiency and in vitro drug release kinetics. The data was analyzed by SPSS 19.0 software package.
The BMP-2 loaded chitosan nanospheres were spherical in shape, smooth on surface and uniform dispersion without aggregation. The mean diameter was 150.85 nm. The dispersion index was 0.37, and zeta potential was +35.42 mV. The average loading efficiency and encapsulation efficiency were (56.83 ± 2.26)% and (68.24 ± 3.83)%, respectively. Release experiment in vitro showed that the releasing property of BMP-2 loaded chitosan nanospheres was consistent with two-phase kinetic regulation and BMP-2 was controlled to release from the chitosan nanospheres over 30 days.
The BMP-2 loaded chitosan nanospheres prepared by ionic crosslinking method are successfully prepared which show a good controlled release property. It provides the basis for further application in bone tissue engineering.
制备用于负载骨形态发生蛋白-2（BMP-2）的壳聚糖纳米球，并评估其尺寸、zeta电位、外观、体外降解及释放特性，进而研究其作为BMP-2缓释载体的可行性。
采用三聚磷酸钠（TPP）与壳聚糖通过离子交联法制备负载BMP-2的壳聚糖纳米球。利用透射电子显微镜评估其形态学特性，用激光粒度分析仪分析粒径、Zeta电位及分布。进行溶菌酶降解实验以评估生物降解行为。采用酶联免疫吸附测定法（ELISA）测定负载效率、包封率及体外药物释放动力学。数据用SPSS 19.0软件包进行分析。
负载BMP-2的壳聚糖纳米球呈球形，表面光滑，分散均匀无聚集。平均直径为150.85 nm。分散指数为0.37，zeta电位为+35.42 mV。平均负载效率和包封率分别为（56.83±2.26）%和（68.24±3.83）%。体外释放实验表明，负载BMP-2的壳聚糖纳米球的释放特性符合双相动力学规律，BMP-2在30天内从壳聚糖纳米球中得到控释。
通过离子交联法成功制备了负载BMP-2的壳聚糖纳米球，其具有良好的控释性能。为其在骨组织工程中的进一步应用提供了依据。
