波兰东北部临床大学医院耐碳青霉烯铜绿假单胞菌分离株中质粒OXA及其他β-内酰胺酶基因的鉴定
Identification of plasmid OXA and other β-lactamase genes among carbapenem-resistant isolates of Pseudomonas aeruginosa from the Clinical University Hospital in northeastern Poland.
作者信息
Sacha Paweł, Michalska Anna, Ojdana Dominika, Wieczorek Piotr, Hauschild Tomasz, Majewski Piotr, Tryniszewska Elżbieta
机构信息
Department of Microbiological Diagnostics and Infectious Immunology, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Bialystok, Poland.
Department of Microbiology, Faculty of Biology and Chemistry, Institute of Biology, University of Bialystok, Poland.
出版信息
New Microbiol. 2015 Apr;38(2):271-5. Epub 2015 Apr 29.
The aim of the study was to evaluate the prevalence of OXA and other β-lactamase genes, antibiotic susceptibility, and the genetic relatedness among clinical isolates of P. aeruginosa resistant to carbapenems. The presence of bla- OXA genes was demonstrated in 48% of isolates belonging to four PFGE profiles. Most of them contained the blaOXA-2 gene (88.3%). Other blaOXA genes (Ps1310 with blaOXA-30 and Ps1309 with blaOXA-10) were found in only two isolates. The tested isolates also contained other β-lactamase genes such as blaVIM-2, blaVIM-4, blaSHV-5, and blaTEM-1. All isolates were susceptible only to colistin (100%).
本研究的目的是评估耐碳青霉烯类铜绿假单胞菌临床分离株中OXA及其他β-内酰胺酶基因的流行情况、抗生素敏感性以及基因相关性。在属于四种脉冲场凝胶电泳（PFGE）图谱的分离株中，48%检测到bla - OXA基因。其中大多数含有blaOXA - 2基因（88.3%）。仅在两株分离株中发现了其他blaOXA基因（携带blaOXA - 30的Ps1310和携带blaOXA - 10的Ps1309）。受试分离株还含有其他β-内酰胺酶基因，如blaVIM - 2、blaVIM - 4、blaSHV - 5和blaTEM - 1。所有分离株仅对黏菌素敏感（100%）。
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