Li Bao, Huang Dake, Gui Li, Qi Weiqin, Jia Xuemei, Lu Baojing
School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2015 May;31(5):581-4.
To investigate the expression profile of tumor necrosis factor α (TNF-α), interleukin 1β (IL-1β) and IL-8 in the hippocampus and cerebral cortex of apolipoprotein E (ApoE) knockout (ApoE-/-) mice.
Twenty 4-week-old male mice were divided into 2 groups: wild-type mice and ApoE-/- mice, 10 mice for each. After 12-week feeding, the blood sample was taken for serum lipid test and brain tissue were obtained for fixation and embedding. The histological changes of the hippocampus and cerebral cordex were observed by HE staining and the expressions of TNF-α, IL-1β and IL-8 proteins were detected by immunohistochemistry.
Compared with the wild-type mice, the numbers of the IL-1β and IL-8-positive cells were markedly elevated in the hippocampus and cerebral cortex in ApoE-/- mice. The number of the TNF-α-positive cells was markedly raised in the cerebral cortex after ApoE knockout, and the intensity of TNF-α positive substances in the hippocampus is higher in ApoE-/- mice than in wild-type mice.
The expressions of IL-1β and IL-8 in the brain increased after ApoE knockout in mice.
研究载脂蛋白E（ApoE）基因敲除（ApoE-/-）小鼠海马和大脑皮质中肿瘤坏死因子α（TNF-α）、白细胞介素1β（IL-1β）和IL-8的表达谱。
将20只4周龄雄性小鼠分为2组：野生型小鼠和ApoE-/-小鼠，每组10只。饲养12周后，采集血样进行血脂检测，并获取脑组织进行固定和包埋。通过苏木精-伊红（HE）染色观察海马和大脑皮质的组织学变化，采用免疫组织化学法检测TNF-α、IL-1β和IL-8蛋白的表达。
与野生型小鼠相比，ApoE-/-小鼠海马和大脑皮质中IL-1β和IL-8阳性细胞数量明显增加。ApoE基因敲除后大脑皮质中TNF-α阳性细胞数量明显增多，且ApoE-/-小鼠海马中TNF-α阳性物质的强度高于野生型小鼠。
小鼠ApoE基因敲除后大脑中IL-1β和IL-8的表达增加。
