Klein C
Reprod Fertil Dev. 2015 May 5. doi: 10.1071/RD14489.
Maintenance of pregnancy is dependent on the exchange of signals between the conceptus and the endometrium. The objective of this study was to use next-generation sequencing to determine transcriptome blueprints of the conceptus and endometrium 16 days after ovulation in the horse. There were 7760 and 10 182 genes expressed in the conceptus and endometrium, respectively, of which 7029 were present in both. Genes related to developmental processes were enriched among conceptus-specific transcripts, whereas many endometrium-specific genes had known roles in cell communication, cell adhesion and response to stimuli. The integrin signalling pathway was overrepresented in both transcriptomes. In that regard, it was hypothesised that integrins ITGA5B1 and ITGAVB3 interact with conceptus-derived fibrinogen, potentially contributing to cessation of conceptus mobility. That several growth factors and their corresponding receptors (e.g. HDGF, NOV, CYR61, CTGF, HBEGF) were expressed by conceptus and endometrium were attributed to cross-talk. In addition, Cytoscape interaction analysis revealed a plethora of interactions between genes expressed by the conceptus and endometrium, during a period when the former had substantial movement within the uterus. This is the first report of concurrent transcriptome analysis of conceptus and endometrium in the mare, with numerous findings to provide rationale for further investigation.
维持妊娠依赖于孕体与子宫内膜之间的信号交换。本研究的目的是使用下一代测序技术来确定马排卵后16天孕体和子宫内膜的转录组蓝图。孕体和子宫内膜中分别有7760个和10182个基因表达，其中7029个基因在两者中都存在。与发育过程相关的基因在孕体特异性转录本中富集，而许多子宫内膜特异性基因在细胞通讯、细胞黏附和对刺激的反应中具有已知作用。整合素信号通路在两个转录组中均过度表达。在这方面，有人提出整合素ITGA5B1和ITGAVB3与孕体来源的纤维蛋白原相互作用，可能有助于孕体停止移动。孕体和子宫内膜均表达几种生长因子及其相应受体（如HDGF、NOV、CYR61、CTGF、HBEGF），这归因于相互作用。此外，Cytoscape相互作用分析显示，在孕体在子宫内有大量移动的时期，孕体和子宫内膜表达的基因之间存在大量相互作用。这是关于母马孕体和子宫内膜同时进行转录组分析的首份报告，有众多发现可为进一步研究提供理论依据。
