在马拉维布兰太尔对有腹泻和无腹泻的住院儿童进行产肠毒素大肠杆菌的检测。
Detection of enterotoxigenic E. coli in hospitalised children with and without diarrhoea in Blantyre, Malawi.
作者信息
Trainor Eamonn, Iturriza-Gómara Miren, Ngwira Bagrey, Cunliffe Nigel
机构信息
a Department of Clinical Infection, Microbiology and Immunology, Institute of Infection and Global Health , University of Liverpool , UK.
b Department of Community Health, College of Medicine , University of Malawi, Blantyre , Malawi.
出版信息
Paediatr Int Child Health. 2016 May;36(2):102-5. doi: 10.1179/2046905515Y.0000000014.
BACKGROUND
Enterotoxigenic E. coli (ETEC) is an important cause of diarrhoea among children in developing countries. The burden of ETEC infection was investigated for the first time in children in Malawi.
METHODS
Faecal samples obtained from children < 5 years of age hospitalised with diarrhoea in Blantyre, Malawi over a 10-year period (1997-2007) before introduction of the rotavirus vaccine were examined by PCR for ETEC heat-labile (LT) and heat-stable (STh and STp) enterotoxins. Children hospitalised without diarrhoea were enrolled over a 2-year period as a comparison group.
RESULTS
ETEC was detected in 205 (10.6%) of 1941 children with diarrhoea. The most prevalent toxin type was STh (6·6%), followed by LT (2·1%) and STp (0·9%). ETEC infection was most prevalent in infants aged 6-11 months. Co-infection with rotavirus was common. ETEC was detected in 37 (7·3%) of 507 children without diarrhoea.
CONCLUSION
The burden of ETEC infection in young Malawian children is substantial and should become a focus of diarrhoea prevention efforts in the post-rotavirus vaccine era.
背景
产肠毒素大肠杆菌（ETEC）是发展中国家儿童腹泻的重要病因。首次在马拉维儿童中调查了ETEC感染的负担。
方法
在引入轮状病毒疫苗之前的10年期间（1997 - 2007年），从马拉维布兰太尔因腹泻住院的5岁以下儿童中获取粪便样本，通过聚合酶链反应（PCR）检测ETEC热不稳定（LT）和热稳定（STh和STp）肠毒素。在2年期间纳入未患腹泻而住院的儿童作为对照组。
结果
在1941例腹泻儿童中，205例（10.6%）检测到ETEC。最常见的毒素类型是STh（6.6%），其次是LT（2.1%）和STp（0.9%）。ETEC感染在6 - 11个月大的婴儿中最为普遍。与轮状病毒的合并感染很常见。在507例未患腹泻的儿童中，37例（7.3%）检测到ETEC。
结论
马拉维幼儿中ETEC感染的负担相当大，应成为轮状病毒疫苗接种后腹泻预防工作的重点。
Zotero 插件