Oshiro Ikuya, Jitsuzaki Daichi, Onizuka Kazumitsu, Nishimoto Atsushi, Taniguchi Yosuke, Sasaki Shigeki
Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582 (Japan).
CREST, Japan Science and Technology Agency, 4-1-8 Motomachi, Kawaguchi, Saitama 332-0012 (Japan).
Chembiochem. 2015 May 26;16(8):1199-204. doi: 10.1002/cbic.201500084. Epub 2015 May 4.
Non-natural RNA modifications have been widely used to study the function and structure of RNA. Expanding the study of RNA further requires versatile and efficient tools for site-specific RNA modification. We recently established a new strategy for the site-specific modification of RNA based on a functionality-transfer reaction between an oligodeoxynucleotide (ODN) probe and an RNA substrate. 2'-Deoxy-6-thioguanosine was used to anchor the transfer group, and the 4-amino group of cytosine or the 2-amino group of guanine was specifically modified. In this study, 2'-deoxy-4-thiothymidine was adopted as a new platform to target the 6-amino group of adenosine. The (E)-pyridinyl vinyl keto transfer group was attached to the 4-thioT in the ODN probe, and it was efficiently and specifically transferred to the 6-amino group of the opposing adenosine in RNA in the presence of CuCl2 . This method expands the available RNA target sites for specific modification.
非天然RNA修饰已被广泛用于研究RNA的功能和结构。进一步拓展RNA研究需要用于位点特异性RNA修饰的通用且高效的工具。我们最近基于寡脱氧核苷酸（ODN）探针与RNA底物之间的功能转移反应，建立了一种用于RNA位点特异性修饰的新策略。2'-脱氧-6-硫代鸟苷用于锚定转移基团，胞嘧啶的4-氨基或鸟嘌呤的2-氨基被特异性修饰。在本研究中，采用2'-脱氧-4-硫代胸苷作为靶向腺苷6-氨基的新平台。（E）-吡啶基乙烯基酮转移基团连接到ODN探针中的4-硫代胸苷上，并且在氯化铜存在下，它被高效且特异性地转移到RNA中相对腺苷的6-氨基上。该方法扩展了可用于特异性修饰的RNA靶位点。
