Aquino Monally C C, Widmer Giovanni, Zucatto Anaiza S, Viol Milena A, Inácio Sandra V, Nakamura Alex A, Coelho Willian M D, Perri Silvia H V, Meireles Marcelo V, Bresciani Katia D S
UNESP, Faculdade de Medicina Veterinária de Araçatuba, Universidade Estadual Paulista Júlio de Mesquita Filho, Araçatuba, São Paulo, Brazil.
Department of Infectious Disease & Global Health, Cummings School of Veterinary Medicine at Tufts University, North Grafton, Massachusetts, USA.
J Eukaryot Microbiol. 2015 Sep-Oct;62(5):657-61. doi: 10.1111/jeu.12223. Epub 2015 May 28.
With the aim of determining the occurrence of Cryptosporidium spp., 222 fecal samples were collected from Murrah buffalo calves aged up to 6 mo. Fecal DNA was genotyped with a nested polymerase chain reaction targeting the 18S rRNA gene and sequencing of the amplified fragment. Nested 18S PCR was positive for 48.2% of the samples. Sequence analysis showed that the most frequent species in these animals was Cryptosporidium ryanae, which was present in buffalo calves as young as 5 d. The zoonotic species Cryptosporidium parvum was detected in one animal. An uncommon Cryptosporidium 18S genotype was found in buffaloes.
为了确定隐孢子虫属的感染情况，从6月龄以下的摩拉水牛犊中采集了222份粪便样本。采用针对18S rRNA基因的巢式聚合酶链反应对粪便DNA进行基因分型，并对扩增片段进行测序。巢式18S PCR检测显示48.2%的样本呈阳性。序列分析表明，这些动物中最常见的物种是雷氏隐孢子虫，在5日龄的水牛犊中就已出现。在一只动物中检测到了人兽共患的微小隐孢子虫。在水牛中发现了一种不常见的隐孢子虫18S基因型。
