Verbeke Joren, Boulougouris Xanthippe, Rogiers Carolien, Burvenich Christian, Peelman Luc, De Spiegeleer Bart, De Vliegher Sarne
M-team and Mastitis and Milk Quality Research Unit, Department of Reproduction, Obstetrics, and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan, 133, Merelbeke, Belgium.
Department of Comparative Physiology and Biometrics, Faculty of Veterinary Medicine, Ghent University, Salisburylaan, 133, Merelbeke, Belgium.
BMC Vet Res. 2015 May 6;11:104. doi: 10.1186/s12917-015-0418-5.
Associations between polymorphisms in the bovine CXCR1 gene, encoding the chemokine (C-X-C motif) receptor 1 (IL8RA), and neutrophil traits and mastitis have been described. In the present study, blood neutrophils were isolated from 20 early lactating heifers with different CXCR1 genotype at position 735 or 980. The cells were incubated with different concentrations of recombinant bovine IL-8 (rbIL-8) for 2 or 6 h and stimulated with phorbol 12-myristate 13-acetate (PMA) or opsonized zymosan particles (OZP). Potential association between CXCR1 genotype and production of reactive oxygen species (ROS) was studied.
Although on single nucleotide polymorphisms (SNPs) may potentially affect CXCR1 function, SNPs c.735C > G and c.980A > G showed no association with ROS production with or without incubation of rbIL-8. Neutrophils incubated with rbIL-8 for 2 or 6 h showed higher PMA- and lower OZP-induced ROS production compared to control without rbIL-8.
In the present study no association could be detected between superoxide production by isolated bovine neutrophils during early lactation and CXCR1 gene polymorphism. IL-8 showed to possess inhibitory effects on ROS generation in bovine neutrophils.
编码趋化因子（C-X-C基序）受体1（IL8RA）的牛CXCR1基因多态性与中性粒细胞性状及乳腺炎之间的关联已见报道。在本研究中，从20头处于泌乳早期、CXCR1基因735或980位点基因型不同的小母牛中分离出血液中性粒细胞。将细胞与不同浓度的重组牛IL-8（rbIL-8）孵育2或6小时，并用佛波醇12-肉豆蔻酸酯13-乙酸酯（PMA）或调理酵母聚糖颗粒（OZP）刺激。研究了CXCR1基因型与活性氧（ROS）产生之间的潜在关联。
尽管单核苷酸多态性（SNP）可能潜在影响CXCR1功能，但SNP c.735C＞G和c.980A＞G与有无rbIL-8孵育情况下的ROS产生均无关联。与未用rbIL-8处理的对照组相比，用rbIL-8孵育2或6小时的中性粒细胞显示出更高的PMA诱导的ROS产生和更低的OZP诱导的ROS产生。
在本研究中，未检测到泌乳早期分离的牛中性粒细胞超氧化物产生与CXCR1基因多态性之间存在关联。IL-8对牛中性粒细胞ROS生成具有抑制作用。
