Ye Jianbin, Xiao Lihua, Wang Yuanfei, Guo Yaqiong, Roellig Dawn M, Feng Yaoyu
State Key Laboratory of Bioreactor Engineering, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai, 200237, China.
Centers for Disease Control and Prevention, Atlanta, Georgia, 30329, USA.
Vet Parasitol. 2015 Jun 15;210(3-4):235-9. doi: 10.1016/j.vetpar.2015.04.011. Epub 2015 Apr 24.
To examine the occurrence and genotype distribution of Giardia duodenalis and Enterocytozoon bieneusi in sheep, fecal specimens were collected from 162 lambs and 213 ewes on seven farms in the Hulunbeier Prairie in Inner Mongolia, China. By PCR analysis of the triose-phosphate isomerase gene, 16 of the 375 (4.3%) specimens were positive for G. duodenalis, with 13 sequenced successfully belonging to assemblage A. Lambs had a significantly higher infection rate than ewes (8.6% versus 0.9%, respectively). The dominance of assemblage A in sheep was supported by PCR analysis of the β-giardin and glutamate dehydrogenase genes; 4 of 7 β-giardin-positive specimens and 3 of 5 glutamate dehydrogenase-positive specimens were identified as assemblage A. A much higher occurrence of E. bieneusi was detected by PCR analysis of the ribosomal internal transcribed spacer; 77.8% of lambs and 62.9% of ewes were positive for the pathogen. Two genotypes of E. bieneusi were found: BEB6 and CM7. Genotype BEB6 was seen in 237 animals and on all seven farms, whereas genotype CM7 was detected in 23 animals from six farms. These data indicate that sheep in Inner Mongolia are commonly infected with G. duodenalis assemblage A and E. bieneusi genotype BEB6, two zoonotic pathogens in China.
为检测内蒙古呼伦贝尔草原7个养殖场162只羔羊和213只母羊中十二指肠贾第虫和微小隐孢子虫的感染情况及基因型分布，采集了粪便样本。通过磷酸丙糖异构酶基因的PCR分析，375份样本中有16份（4.3%）十二指肠贾第虫呈阳性，其中13份成功测序，属于A群。羔羊的感染率显著高于母羊（分别为8.6%和0.9%）。β-贾第虫蛋白和谷氨酸脱氢酶基因的PCR分析支持了A群在绵羊中的优势地位；7份β-贾第虫蛋白阳性样本中有4份，5份谷氨酸脱氢酶阳性样本中有3份被鉴定为A群。通过核糖体内部转录间隔区的PCR分析检测到微小隐孢子虫的感染率更高；77.8%的羔羊和62.9%的母羊该病原体呈阳性。发现了微小隐孢子虫的两种基因型：BEB6和CM7。在237只动物中以及所有7个养殖场均发现了基因型BEB6，而在6个养殖场的23只动物中检测到了基因型CM7。这些数据表明，中国内蒙古的绵羊普遍感染十二指肠贾第虫A群和微小隐孢子虫基因型BEB6这两种人兽共患病原体。
