Bruns T, Schickinger S, Schneckenburger H
Aalen University, Institute of Applied Research, Beethovenstr. 1, 73430, Aalen, Germany.
J Microsc. 2015 Oct;260(1):30-6. doi: 10.1111/jmi.12263. Epub 2015 May 6.
In common light microscopy, observation of samples is only possible from one perspective. However, especially for larger three-dimensional specimens observation from different views is desirable. Therefore, we are presenting a sample holder permitting rotation of the specimen around an axis perpendicular to the light path of the microscope. Thus, images can be put into a defined multidimensional context, enabling reliable three-dimensional reconstructions. The device can be easily adapted to a great variety of common light microscopes and is suitable for various applications in science, education and industry, where the observation of three-dimensional specimens is essential. Fluorescence z-projection images of copepods and ixodidae ticks at different rotation angles obtained by confocal laser scanning microscopy and light sheet fluorescence microscopy are reported as representative results.
在普通光学显微镜下，只能从一个视角观察样本。然而，特别是对于较大的三维标本，从不同视角进行观察是很有必要的。因此，我们展示了一种样品架，它能使标本绕垂直于显微镜光路的轴旋转。这样，图像就能被置于一个明确的多维背景中，从而实现可靠的三维重建。该装置可以很容易地适配到各种常见的光学显微镜上，适用于科学、教育和工业等各种领域中对三维标本进行观察至关重要的各种应用。通过共聚焦激光扫描显微镜和光片荧光显微镜获得的不同旋转角度下桡足类动物和硬蜱的荧光z投影图像被作为代表性结果进行了报道。
