Henry K D, Williams E R, Wang B H, McLafferty F W, Shabanowitz J, Hunt D F
Department of Chemistry, Baker Laboratory, Cornell University, Ithaca, NY 14853.
Proc Natl Acad Sci U S A. 1989 Dec;86(23):9075-8. doi: 10.1073/pnas.86.23.9075.
The multiply charged ions produced by electrospray ionization of peptides of molecular masses up to 29 kDa have been successfully introduced into a Fourier transform mass spectrometer of unique capabilities for tandem mass spectrometry, large ion dissociation, and resolution. Electrospray ionization places an unusually high number of charges on a peptide yielding mass/charge (m/z) values of 600-1500; in this range at normal operating pressures (approximately 10(-9) torr; 1 torr = 133.3 Pa) Fourier-transform mass spectrometry resolving power is greater than 100,000. Although only 10(-7) torr pressure has been obtained with the initial interface, the resulting resolving power of 5000 makes possible the resolution of isotopic peaks of multiply charged ions. Mass measuring accuracies of a few daltons for molecular masses up to 17 kDa have also been achieved.
分子量高达29 kDa的肽通过电喷雾电离产生的多电荷离子已成功引入一台具有串联质谱分析、大离子解离和高分辨率等独特功能的傅里叶变换质谱仪中。电喷雾电离会在肽上产生异常多的电荷，从而得到质荷比（m/z）为600 - 1500的值；在这个范围内，在正常操作压力下（约10⁻⁹ 托；1托 = 133.3帕），傅里叶变换质谱仪的分辨率大于100,000。虽然最初的接口仅获得了10⁻⁷ 托的压力，但由此产生的5000的分辨率使得多电荷离子的同位素峰得以分辨。对于分子量高达17 kDa的分子质量，还实现了几道尔顿的质量测量精度。
