用于四倍体互补试验的小鼠胚胎融合。

Mouse embryos' fusion for the tetraploid complementation assay.

作者信息

Gertsenstein Marina

机构信息

Toronto Centre for Phenogenomics (TCP), Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, 25 Orde, Toronto, ON, Canada, M5T 3H7,

出版信息

Methods Mol Biol. 2015;1313:41-59. doi: 10.1007/978-1-4939-2703-6_3.

DOI:10.1007/978-1-4939-2703-6_3

PMID:25947655

Abstract

Production of the germline-competent chimeras using genetically modified ES cell lines is an essential step in the establishment of novel mouse models. In addition chimeras provide a powerful tool to study the cell lineage and to analyze complex phenotypes of mutant mice. Mouse chimeras with tetraploid embryos are used to rescue extraembryonic defects, to analyze an impact of gene function on specific lineage, to study the interaction between embryonic and extraembryonic tissues, and to produce mutant embryos and mice for the phenotype analysis. Tetraploid embryos are generated by the fusion of two blastomeres of the mouse embryo. The applications of tetraploid complementation assay and the protocol are described below.

摘要

使用基因工程改造的胚胎干细胞系产生具有生殖系能力的嵌合体是建立新型小鼠模型的关键步骤。此外，嵌合体为研究细胞谱系和分析突变小鼠的复杂表型提供了强大的工具。具有四倍体胚胎的小鼠嵌合体用于挽救胚胎外缺陷、分析基因功能对特定谱系的影响、研究胚胎组织与胚胎外组织之间的相互作用，以及产生用于表型分析的突变胚胎和小鼠。四倍体胚胎通过小鼠胚胎的两个卵裂球融合产生。以下描述四倍体互补分析的应用及方案。