Kim Eun-Jung, Lee Jong-Min, Jung Han-Sung
Division in Anatomy and Developmental Biology, Department of Oral Biology, Research Center for Orofacial Hard Tissue Regeneration, Brain Korea 21 Project, Oral Science Research Center, College of Dentistry, Yonsei University, Seoul 120-752, Republic of Korea.
Division in Anatomy and Developmental Biology, Department of Oral Biology, Research Center for Orofacial Hard Tissue Regeneration, Brain Korea 21 Project, Oral Science Research Center, College of Dentistry, Yonsei University, Seoul 120-752, Republic of Korea ; Oral Biosciences, Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR.
Dev Reprod. 2013 Sep;17(3):215-20. doi: 10.12717/DR.2013.17.3.215.
Recently, the RNA/DNA-binding protein FUS, Fused in sarcoma, was shown to play a role in growth, differentiation, and morphogenesis in vertebrates. Because little is known about Fus, we investigated its expression pattern in murine tooth development. In situ hybridization of mouse mandibles at specific developmental stages was performed with a DIG-labeled RNA probe. During early tooth development, Fus was detected in the dental epithelium and dental mesenchyme at 11 days postcoitum (dpc) and 12 dpc. From 14 dpc, Fus was strongly expressed in the dental papilla and the cervical loop of the dental epithelium. At postnatal day 4 (PN4), Fus expression was observed in the odontoblasts, ameloblasts, the proliferation zone of the pulp, and the cervical loop. At PN14, the expression pattern of Fus was found to be maintained in the odontoblasts and the proliferation zone of the pulp. Furthermore, Fus expression was especially strong in the Hertwig's epithelial root sheath (HERS). Therefore, this study suggests that Fus may play a role in the HERS during root development.
最近，RNA/DNA结合蛋白FUS（肉瘤融合蛋白）被证明在脊椎动物的生长、分化和形态发生中发挥作用。由于对Fus了解甚少，我们研究了它在小鼠牙齿发育中的表达模式。用DIG标记的RNA探针在特定发育阶段对小鼠下颌骨进行原位杂交。在牙齿发育早期，在妊娠后11天（dpc）和12 dpc时，在牙上皮和牙间充质中检测到Fus。从14 dpc开始，Fus在牙乳头和牙上皮的颈环中强烈表达。在出生后第4天（PN4），在成牙本质细胞、成釉细胞、牙髓增殖区和颈环中观察到Fus表达。在PN14时，发现Fus的表达模式在成牙本质细胞和牙髓增殖区中得以维持。此外，Fus在赫特维希上皮根鞘（HERS）中的表达尤为强烈。因此，本研究表明Fus可能在牙根发育过程中的HERS中发挥作用。
