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韩国和中国带鱼(Trichiurus lepturus)种群之间的遗传变异

Genetic Variations between Hairtail (Trichiurus lepturus) Populations from Korea and China.

作者信息

Yoon Jong-Man

机构信息

Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University, Gunsan 573-701, Republic of Korea.

出版信息

Dev Reprod. 2013 Dec;17(4):363-7. doi: 10.12717/DR.2013.17.4.363.

Abstract

PCR analysis generated on the genetic data showed that the geographic hairtail (Trichiurus lepturus) population from Korea in the Yellow Sea was more or less separated from geographic hairtail population from China in the South Sea. The average bandsharing value (mean±SD) within hairtail population from Korea showed 0.859±0.031, whereas 0.752±0.039 within population from China. Also, bandsharing values between two hairtail populations ranged from 0.470 to 0.611, with an average of 0.542±0.059. As compared separately, the bandsharing values of individuals within hairtail population from Korea were comparatively higher than those of individuals within population from China. The hierarchical dendevrepogram resulted from reliable oligonucleotides primers, indicating two genetic clusters composed of cluster 1 (KOREANHAIR1KOREANHAIR11) and cluster 2 (CHINESEHAI12CHINESEHAI22). The genetic distances between two geographic populations ranged from 0.038 to 0.476. Individual No. 11 within hairtail population from Korea was genetically closely related with No. 10 (genetic distance=0.038). The longest genetic distance (0.476) displaying significant molecular difference was also between individual No. 01 within hairtail population from Korea and No. 22 from Chinese. In the present study, PCR analysis has revealed significant genetic distances between two hairtail population pairs (P<0.05).

摘要

对遗传数据进行的聚合酶链反应(PCR)分析表明,黄海韩国海域的竹荚鱼(Trichiurus lepturus)种群与南海中国海域的竹荚鱼种群或多或少有所分离。韩国竹荚鱼种群内的平均条带共享值(均值±标准差)为0.859±0.031,而中国种群内为0.752±0.039。此外,两个竹荚鱼种群之间的条带共享值在0.470至0.611之间,平均为0.542±0.059。单独比较时,韩国竹荚鱼种群内个体的条带共享值相对高于中国种群内个体的条带共享值。由可靠的寡核苷酸引物得出的层次聚类树状图表明,存在两个遗传簇,即簇1(KOREANHAIR1KOREANHAIR11)和簇2(CHINESEHAI12CHINESEHAI22)。两个地理种群之间的遗传距离在0.038至0.476之间。韩国竹荚鱼种群中的个体11与个体10在遗传上密切相关(遗传距离=0.038)。显示出显著分子差异的最长遗传距离(0.476)也存在于韩国竹荚鱼种群中的个体01与中国的个体22之间。在本研究中,PCR分析揭示了两对竹荚鱼种群之间存在显著的遗传距离(P<0.05)。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e5a/4382950/29d602a4cee8/devrep-17-363-F1.jpg

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