Baek Hea Ja, Kim Dea Geun, Kim Hyung Bae
Department of Marine Biology, Pukyong National University, Busan 608-737, Republic of Korea.
Department of Marine Bio-Resources, Gangwon Provincial University, Gangnung 210-804, Republic of Korea.
Dev Reprod. 2013 Dec;17(4):421-6. doi: 10.12717/DR.2013.17.4.421.
In this study, oocyte steroidogenesis are investigated in relation to oocyte development in the starry flounder, Platichthys stellatus, a marine multiple spawner. Vitellogenic (0.52 and 0.55 mm oocyte diameter) and mature oocytes (0.63, 0.66 and 0.71 mm oocyte diameter) were incubated in vitro in the presence of [(3)H]17α-hydevrepoxyprogesterone ([(3)H]17α-OHP) as a precursor. Steroid metabolites were extracted from the incubated media and oocytes, the extracts were separated and identified by thin-layer chromatography (TLC), high performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS). The major metabolites produced from [(3)H]17α-OHP were andevrepogens [andevrepostenedione (A4) and testosterone (T)] and estrogens [17β-estradiol (E2) and estrone (E1)] and progestins [17α,20α-dihydevrepoxy-4-pregnen-3-one (17α20αP) and 17α,20β-dihydevrepoxy-4-pregnen-3-one (17α20βP)] in vitellogenic and mature oocytes. The results from this study suggest the potential roles of E1 in the oocytes with diameter 0.52-0.71 mm, 17α20αP and 17α20βP at the oocytes of 0.63, 0.66 and 0.71 mm.
在本研究中，对多产的海水鱼类星斑川鲽（Platichthys stellatus）卵母细胞的类固醇生成与卵母细胞发育的关系进行了研究。将卵黄生成期（卵母细胞直径为0.52和0.55毫米）和成熟卵母细胞（卵母细胞直径为0.63、0.66和0.71毫米）在体外与作为前体的[(3)H]17α-羟基脱氢表雄酮（[(3)H]17α-OHP）一起孵育。从孵育培养基和卵母细胞中提取类固醇代谢物，提取物通过薄层色谱法（TLC）、高效液相色谱法（HPLC）和气相色谱-质谱联用法（GC-MS）进行分离和鉴定。[(3)H]17α-OHP产生的主要代谢物在卵黄生成期和成熟卵母细胞中为雄激素[脱氢表雄烯二酮（A4）和睾酮（T）]、雌激素[17β-雌二醇（E2）和雌酮（E1）]以及孕激素[17α,20α-二羟基脱氢表雄烯-4-孕烯-3-酮（17α20αP）和17α,20β-二羟基脱氢表雄烯-4-孕烯-3-酮（17α20βP）]。本研究结果表明，E1在直径为0.52 - 0.71毫米的卵母细胞中、17α20αP和17α20βP在直径为0.63、0.66和0.71毫米的卵母细胞中具有潜在作用。
