Cho Hyun Chul, Kim Ji Eun, Kim Hyung Bae, Baek Hea Ja
Dept. of Marine Biology, Pukyong National University, Busan 608-737, Korea.
Dept. of Marine Bio-Resources, Gangwon Provincial University, Gangnung 210-804, Korea.
Dev Reprod. 2015 Mar;19(1):19-24. doi: 10.12717/devrep.2015.19.1.019.
This study was conducted to determine the stress response [ethological (operculum movement number (OMN)), hematological (hematocrit and hemoglobin), biochemical (glucose, cortisol and glutamic oxaloacetic transaminase (GOT))] in red spotted grouper, Epinephelus akaara during exposure of different water temperature in winter season. This species (Total length, 18.56±0.34 cm) previously maintained in water temperature of 15°C were transferred to 15, 20 and 25°C. During experimental period (7 days), OMN, hematocrit (Ht), glucose and GOT values were significantly high in 15°C when compared to 20 and 25°C. Hemoglobin value was also increased at 15°C, but no significant differences. There was no differences in cortisol levels among the temperature groups. No fish mortality was observed during the experimental period. From these results, 15°C is likely more stressful to red spotted grouper than 20°C and 25°C. These observations confirm that red spotted grouper adapts better to temperatures between 20 and 25°C during the winter season.
本研究旨在确定冬季不同水温暴露期间红斑石斑鱼（Epinephelus akaara）的应激反应[行为学指标（鳃盖运动次数（OMN））、血液学指标（血细胞比容和血红蛋白）、生化指标（葡萄糖、皮质醇和谷草转氨酶（GOT））]。将先前饲养在15°C水温中的该物种（全长18.56±0.34厘米）转移至15°C、20°C和25°C水温环境。在实验期间（7天），与20°C和25°C相比，15°C时的OMN、血细胞比容（Ht）、葡萄糖和GOT值显著更高。15°C时血红蛋白值也有所升高，但无显著差异。各温度组之间的皮质醇水平无差异。实验期间未观察到鱼死亡。从这些结果来看，15°C对红斑石斑鱼的压力可能比20°C和25°C更大。这些观察结果证实，冬季红斑石斑鱼更能适应20°C至25°C之间的水温。
