Halldorsdottir S, Larsen H J, Mehl R
Norwegian College of Veterinary Medicine, Department of Microbiology, Oslo.
Res Vet Sci. 1989 Nov;47(3):283-7.
Twenty-three Icelandic horses were challenged with extracts of four species of biting midges: Culicoides pulicaris, C chiopterus, C obsoletus and C impunctatus. Fourteen of the tested horses were affected with summer eczema. The horses were challenged intradermally with 0.1 ml of whole-body extracts of midges at a concentration of 0.01 or 0.005 per cent weight/volume. The skin reactions were measured after 30 minutes, 60 or 180 minutes and four, 24 and 48 hours after injection. Antigen titration showed that the reaction was dependent on the antigen concentration. Eight of nine unaffected horses failed to respond to any of the four antigens; the remaining animal responding to two of the four antigens. Ten of the 14 affected horses responded to at least three of the four antigens, while two of the animals in this group failed to respond to any. The mean responses to C chiopterus, C obsoletus and C impunctatus, read after 30 minutes, 60 minutes and four hours were significantly higher in the affected horses than in the unaffected horses. A significant difference was also found in the mean response to C chiopterus and C impunctatus, read after 24 hours.
用四种蠓的提取物对23匹冰岛马进行了攻毒试验，这四种蠓分别是：淡黄库蠓、毛蠓、废弃库蠓和无 punctatus。14匹受试马患有夏季湿疹。用浓度为0.01%或0.005%重量/体积的0.1毫升蠓全身体提取物对马进行皮内攻毒。在注射后30分钟、60或180分钟以及4、24和48小时后测量皮肤反应。抗原滴定表明反应取决于抗原浓度。9匹未受影响的马中有8匹对四种抗原中的任何一种都没有反应；其余1只动物对四种抗原中的两种有反应。14匹受影响的马中有10匹对四种抗原中的至少三种有反应，而该组中有2只动物对任何抗原都没有反应。在30分钟、60分钟和4小时后读取的受影响马对毛蠓、废弃库蠓和无 punctatus的平均反应显著高于未受影响的马。在24小时后读取的对毛蠓和无 punctatus的平均反应也发现有显著差异。
