Telethon Institute of Genetics and Medicine, Via Campi Flegrei, 34, 80078 Pozzuoli (Naples), Italy.
Division of Nephrology and Dialysis, Bambino Gesù Children's Hospital - Scientific Institute, Piazza Sant'Onofrio, 4, 00165 Rome, Italy.
Curr Opin Cell Biol. 2015 Aug;35:43-50. doi: 10.1016/j.ceb.2015.04.001. Epub 2015 May 4.
Although they were identified as long ago as the 1960s, there are still many unknowns regarding the functions and composition of membrane contact sites between the endoplasmic reticulum (ER) and the trans-Golgi (TG). While it seems to be fairly well established that they facilitate lipid exchange between the two organelles, much less is known about how they are regulated. A bottleneck in the study of the ER-TG contact sites has been the absence of methods for their biochemical isolation and visualization by light microscopy. Herein we provide an overview of current knowledge about ER-TG contact sites with a particular emphasis on the questions that remain to be explored.
尽管内质网（ER）和反高尔基网络（TG）之间的膜接触位点早在 20 世纪 60 年代就被发现，但关于它们的功能和组成仍然有很多未知。虽然它们促进两个细胞器之间的脂质交换似乎已经得到了很好的证实，但关于它们如何被调控的了解却很少。ER-TG 接触位点研究的一个瓶颈是缺乏用于其生化分离和光镜可视化的方法。本文综述了目前关于 ER-TG 接触位点的知识，特别强调了仍有待探索的问题。
