Wang Yu-Jie, Zhou Si-Min, Xu Gang, Gao Yu-Qi
College of Ethnomedicine, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
College of High Altitude Military Medicine, Third Military Medical University, Chongqing 400038, China.
Molecules. 2015 May 5;20(5):8060-71. doi: 10.3390/molecules20058060.
The MTT assay, as a screening method, has been widely used to measure the viability and proliferation of cells. However, it should be noted that MTT assay may not accurately reflect the effect of Cistanche tubulosa ethanolic extract on EA.hy926 cells viability. To investigate and identity the components responsible for the contradictory observations of the MTT assay, echinacoside and acteoside, two main phenylethanoid glycosides, from C. tubulosa ethanolic extract were isolated. The data derived from CCK-8, Hoechst 33342 and annexin V-FITC/PI assays suggest that the caffeyl group present in both isolated compounds was responsible for the conflicting results of the MTT assay. These data emphasize the need of using a variety of different methods to determine the effect of medicinal agents on cell viability to avoid generating misleading results.
MTT 法作为一种筛选方法，已被广泛用于检测细胞的活力和增殖。然而，应当注意的是，MTT 法可能无法准确反映管花肉苁蓉乙醇提取物对 EA.hy926 细胞活力的影响。为了研究并确定导致 MTT 法出现矛盾观察结果的成分，从管花肉苁蓉乙醇提取物中分离出了两种主要的苯乙醇苷类化合物，松果菊苷和毛蕊花糖苷。来自 CCK-8、Hoechst 33342 和 Annexin V-FITC/PI 检测的数据表明，两种分离出的化合物中存在的咖啡酰基是导致 MTT 法结果矛盾的原因。这些数据强调了需要使用多种不同方法来确定药物对细胞活力的影响，以避免产生误导性结果。
