Nguyen-Tan J Q, Thompson J T
Department of Ophthamology and Visual Science, Yale University, New Haven, Connecticut.
Retina. 1989;9(3):203-9.
Retinal pigment epithelial (RPE) cells were obtained from human cadaver eyes and grown to confluence in 16 mm wells. A sterile intact rabbit vitreous body was isolated and placed on the RPE monolayer. RPE cells with fibrocyte-like morphology appeared over the RPE monolayer within 4 to 6 days. These rapidly proliferating cells were observed to extend cellular processes and migrate into the vitreous body. Migration of the RPE cells progressed over 20 to 40 days, at which time bipolar RPE cells were found in multiple layers of the vitreous body. The contractile properties of the migrating RPE cells were evident as 24% of vitreous bodies contracted into a dense cellular gel. This study provides a model of migration of RPE cells in the vitreous and allows study of the interaction of RPE with the intact vitreous gel.
视网膜色素上皮（RPE）细胞取自人尸体眼睛，并在16毫米孔中培养至汇合。分离出无菌完整的兔玻璃体并置于RPE单层上。在4至6天内，RPE单层上出现了具有成纤维细胞样形态的RPE细胞。观察到这些快速增殖的细胞伸出细胞突起并迁移到玻璃体内。RPE细胞的迁移在20至40天内进行，此时在玻璃体的多层中发现了双极RPE细胞。迁移的RPE细胞的收缩特性很明显，因为24%的玻璃体收缩成致密的细胞凝胶。这项研究提供了一个RPE细胞在玻璃体内迁移的模型，并允许研究RPE与完整玻璃体凝胶的相互作用。
