Lin Chin-Chung, Chen Ching-Chun, Kuo Yueh-Hsiung, Kuo Jong-Tar, Senthil Kumar K J, Wang Sheng-Yang
Taiwan Leader Biotech Company, Taipei, Taiwan.
J Nat Med. 2015 Oct;69(4):513-21. doi: 10.1007/s11418-015-0916-6. Epub 2015 May 8.
Antrodia cinnamomea is a valuable and unique edible fungus originating from the forests of Taiwan. In this study, an anti-metastatic compound, 2,3,5-trimethoxy-4-cresol (TMC), was isolated from the solid-state cultured mycelium of A. cinnamomea. According to the results obtained from cell wound healing, cell migration and invasion assays, TMC effectively suppressed movement, migration and invasion of lung cancer cells at the dosage of 5-40 μM, which was non-toxic to A549 cells. In addition, TMC reduced protein expression of Akt, MMP-2 and MMP-9 and enhanced E-cadherin and TIMP-1 protein expression, which are known to regulate cell adhesion, migration and invasion. Taken together, TMC effectively suppresses movement, migration and invasion of lung cancer cells, and achieves an anti-cancer metastasis effect.
樟芝是一种珍贵且独特的食用真菌，原产于台湾的森林。在本研究中，从樟芝固态培养菌丝体中分离出一种抗转移化合物2,3,5-三甲氧基-4-甲酚（TMC）。根据细胞伤口愈合、细胞迁移和侵袭试验结果，TMC在5-40μM剂量下可有效抑制肺癌细胞的运动、迁移和侵袭，且对A549细胞无毒。此外，TMC降低了Akt、MMP-2和MMP-9的蛋白表达，并增强了E-钙黏蛋白和TIMP-1蛋白表达，已知这些蛋白可调节细胞黏附、迁移和侵袭。综上所述，TMC可有效抑制肺癌细胞的运动、迁移和侵袭，实现抗癌转移效果。
