离子交换色谱法测定血浆中的同型半胱氨酸。

Determination of homocysteine in plasma by ion-exchange chromatography.

作者信息

Andersson A, Brattström L, Isaksson A, Israelsson B, Hultberg B

机构信息

Department of Clinical Chemistry, University Hospital, Lund, Sweden.

出版信息

Scand J Clin Lab Invest. 1989 Sep;49(5):445-9. doi: 10.1080/00365518909089120.

DOI:10.1080/00365518909089120

PMID:2595241

Abstract

A rapid ion-exchange chromatographic method for determination of total homocysteine in plasma is presented. The method has a coefficient of variation of approximately 5%. A comparison with a slower method previously used in our laboratory showed a high correlation (r = 0.993). The risk of oxidation of homocysteine during the chromatography and the necessary level of dithiotreithol to prevent oxidation are also established.

摘要

本文介绍了一种用于测定血浆中总同型半胱氨酸的快速离子交换色谱法。该方法的变异系数约为5%。与我们实验室之前使用的较慢方法进行比较，结果显示相关性很高（r = 0.993）。同时还确定了同型半胱氨酸在色谱分析过程中的氧化风险以及防止氧化所需的二硫苏糖醇水平。