RIKILT - Institute of Food Safety, Wageningen UR (University & Research centre), P.O. Box 230, 6700 AE Wageningen, The Netherlands.
NutriControl, P.O. Box 107, 5460 AC Veghel, The Netherlands.
Food Chem. 2015 Oct 15;185:333-9. doi: 10.1016/j.foodchem.2015.03.107. Epub 2015 Apr 2.
In order to facilitate safe re-introduction of non-ruminant processed animal proteins (PAPs) in aqua feed, two immunoassays have been tested in an interlaboratory study for their capability to detect ruminant PAPs processed under European conditions. The sensitivity of the MELISA-TEK assay was improved by applying a specific extraction kit. Six approved blank pork and poultry samples were adulterated to produce 15 samples spiked at 0.5%, 1.0% and 2.0% with ruminant material, sterilised at either 133 °C or 137 °C. Fourteen participants investigated the 6 blanks and 15 spiked samples, making 21 samples for the final test. For both assays specificity and sensitivity were at 97% or higher. Concordance and accordance were higher than 95% with one exception. The results indicate that both assays provided correct results at 0.5% and higher for the detecting ruminant PAPs (sterilised at 133 °C) in non-ruminant PAPs. Given the 2% upper limit of ruminant PAPs in non-ruminant PAPs for avoiding an increase in BSE incidents, these methods are fit for monitoring non-ruminant PAPs intended for aqua feed.
为了促进非反刍动物加工动物蛋白（PAP）在水产饲料中的安全再引入，在一项实验室间研究中测试了两种免疫测定法，以评估它们检测在欧洲条件下加工的反刍动物 PAP 的能力。通过应用特定的提取试剂盒，改进了 MELISA-TEK 测定法的灵敏度。将六种经批准的空白猪肉和家禽样品掺假，以 0.5%、1.0%和 2.0%的比例用反刍动物材料制成 15 个加标样品，并在 133°C 或 137°C 下进行灭菌。14 名参与者研究了 6 个空白和 15 个加标样品，共进行了 21 个最终测试。两种测定法的特异性和灵敏度均在 97%或更高。除了一个例外，一致性和一致性都高于 95%。结果表明，对于在非反刍动物 PAP 中检测（在 133°C 下灭菌）的反刍动物 PAP，两种测定法在 0.5%及以上均能提供正确的结果。鉴于非反刍动物 PAP 中反刍动物 PAP 的上限为 2%，以避免疯牛病事件的增加，这些方法适用于监测用于水产饲料的非反刍动物 PAP。
