水浸应激对大鼠胃黏膜黏液糖蛋白合成的影响。
Influence of water-immersion stress on synthesis of mucus glycoprotein in the rat gastric mucosa.
作者信息
Tsukada H, Seino Y, Ueda S, Uchino H, Sakai M
机构信息
Division of Metabolism and Clinical Nutrition, Department of Internal Medicine, Kyoto University School of Medicine, Japan.
出版信息
Scand J Gastroenterol Suppl. 1989;162:19-22. doi: 10.3109/00365528909091115.
Gastric mucous cells of rats subjected to water-immersion stress were incubated with [3H]-palmitic acid and [14C]-N-acetylgalactosamine. The peptidyl-tRNA released by gastric polysomes was precipitated with cold ethanol and then the content was determined. A 70% reduction in the peptidyl-tRNA isolated was observed in the stressed rats, as compared with in control rats. The peptide recovered from the peptidyl-tRNA showed 30-50% less [3H]-palmitic acid and [14C]-N-acetylgalactosamine incorporation in the stressed rats than in normal controls. It was thus suggested that translation, acylation and glycosylation of the peptides in the ribosomes of the gastric mucosa were remarkably affected by the stress.
将遭受水浸应激的大鼠胃黏液细胞与[3H] - 棕榈酸和[14C] - N - 乙酰半乳糖胺一起孵育。胃多聚核糖体释放的肽基 - tRNA用冷乙醇沉淀，然后测定其含量。与对照大鼠相比，应激大鼠中分离出的肽基 - tRNA减少了70%。从肽基 - tRNA中回收的肽显示，应激大鼠中[3H] - 棕榈酸和[14C] - N - 乙酰半乳糖胺的掺入量比正常对照少30 - 50%。因此表明，胃黏膜核糖体中肽的翻译、酰化和糖基化受到应激的显著影响。
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