水浸应激诱导的大鼠胃溃疡模型血清血小板活化因子乙酰水解酶活性

Serum platelet-activating factor acetylhydrolase activity in rats with gastric ulcers induced by water-immersion stress.

作者信息

Fujimura K, Sugatani J, Miwa M, Mizuno T, Sameshima Y, Saito K

机构信息

Third Department of Internal Medicine, Kansai Medical School, Osaka, Japan.

出版信息

Scand J Gastroenterol Suppl. 1989;162:59-62. doi: 10.3109/00365528909091125.

DOI:10.3109/00365528909091125

PMID:2595308

Abstract

Platelet-activating factor (PAF) acetylhydrolase is an enzyme which hydrolyzes PAF to yield inactive lysoPAF. This study focused on the influence of water-immersion stress on serum PAF acetylhydrolase activity. The enzyme activity was determined by measurement of [3H]acetate produced from 1-O-alkyl-2-[3H]acetyl-sn-glycero-3-phosphocholine upon precipitation of the complex of the radioactive substrate and albumin with trichloroacetic acid. The onset of water-immersion stress caused the development of gastric lesions associated with a significant increase in serum PAF acetylhydrolase activity. Serum PAF acetylhydrolase may leak into the blood from some tissues in rats with gastric injury induced by water-immersion stress and might control the action of PAF.

摘要

血小板活化因子（PAF）乙酰水解酶是一种将PAF水解生成无活性溶血PAF的酶。本研究聚焦于水浸应激对血清PAF乙酰水解酶活性的影响。通过测量用三氯乙酸沉淀放射性底物与白蛋白的复合物后，由1-O-烷基-2-[3H]乙酰-sn-甘油-3-磷酸胆碱产生的[3H]乙酸来测定酶活性。水浸应激的开始导致胃损伤的出现，并伴有血清PAF乙酰水解酶活性显著增加。血清PAF乙酰水解酶可能从水浸应激诱导胃损伤的大鼠的某些组织中漏入血液，并且可能控制PAF的作用。