Zhu Xiaoming, Yang Yang, Han Tao, Yin Guowu, Gao Ping, Ni Yunfeng, Su Xiaohua, Liu Yuying, Yao Yuanqing
Department of Obstetrics and Gynecology, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shanxi 710038, P.R. China.
Department of Obstetrics and Gynecology, Affiliated Hospital of Xi'an Medical University, Xi'an, Shanxi 710077, P.R. China.
Mol Med Rep. 2015 Aug;12(2):2701-6. doi: 10.3892/mmr.2015.3724. Epub 2015 May 4.
The purpose of the present study was to gain further understanding of the function of microRNA-18a (miR-18a) expression in the JEG-3 human trophoblast cell line. JEG-3 cells were transfected with pre-miR-18a mimics or miR-18a inhibitors. The effects of miR-18a on trophoblast cell invasion and apoptosis, and on the expression of estrogen receptor α (ESRα) were analyzed using a Transwell invasion assay, flow cytometry, reverse transcription-polymerase chain reaction, western blot analysis and a luciferase assay. The results of the present study suggested that miR-18a expression suppression led to a decrease in JEG-3 cell invasion and an increase in JEG-3 cell apoptosis, by inducing ESRα expression. The present study provides evidence for the involvement of miR-18a in the pathogenesis of pre-eclamptic pregnancies.
本研究的目的是进一步了解微小RNA-18a（miR-18a）在JEG-3人滋养层细胞系中的表达功能。用pre-miR-18a模拟物或miR-18a抑制剂转染JEG-3细胞。使用Transwell侵袭试验、流式细胞术、逆转录-聚合酶链反应、蛋白质免疫印迹分析和荧光素酶测定法，分析miR-18a对滋养层细胞侵袭和凋亡以及雌激素受体α（ESRα）表达的影响。本研究结果表明，miR-18a表达抑制通过诱导ESRα表达导致JEG-3细胞侵袭减少和JEG-3细胞凋亡增加。本研究为miR-18a参与子痫前期妊娠的发病机制提供了证据。
