Yilmaz Meral, Sari Ismail, Bagci Binnur, Gumus Erkan, Ozdemir Ozturk
Department of Research Centre, Cumhuriyet University, School of Medicine, Sivas, Turkey.
Iran J Kidney Dis. 2015 May;9(3):209-14.
It has been shown that gene polymorphisms influence the development and progression of chronic kidney disease (CKD). Many studies have indicated that aldosterone synthase CYP11B2 gene polymorphism (-344C>T) influences the aldosterone level, urinary aldosterone excretion, blood pressure, and left ventricular size and mass. We aimed to investigate whether there is an effect of CYP11B2 -344 C>T polymorphism on the development of CKD in a Turkish population.
A total of 240 patients with stage 5 CKD and 240 age- and sex-matched healthy individuals were included in the study. Genotyping of CYP11B2 gene -344 T>C promoter polymorphism was carried out using polymerase chain reaction and restriction fragment length polymorphism methods.
No significant differences were found in the genotype distribution of CYP11B2 -344 C>T polymorphism between the patients and controls; however, -344 C>T polymorphism was significantly more frequent among the CKD patients with diabetes mellitus as compared to those with it (P = .02). Diabetic CKD patients with TC genotype had a 2-fold increased risk for development of the disease than the CKD patients without diabetes mellitus (odds ratio, 2.21; 95% confidence interval, 1.04 to 4.67).
Our study suggests that the CYP11B2 gene -344 C>T polymorphism may have an effect on the development of CKD in diabetic patients.
研究表明基因多态性会影响慢性肾脏病（CKD）的发生和发展。许多研究表明醛固酮合成酶CYP11B2基因多态性（-344C>T）会影响醛固酮水平、尿醛固酮排泄、血压以及左心室大小和质量。我们旨在研究CYP11B2 -344 C>T多态性对土耳其人群CKD发生是否有影响。
本研究纳入了240例5期CKD患者和240例年龄及性别匹配的健康个体。采用聚合酶链反应和限制性片段长度多态性方法对CYP11B2基因-344 T>C启动子多态性进行基因分型。
患者与对照组之间CYP11B2 -344 C>T多态性的基因型分布无显著差异；然而，与无糖尿病的CKD患者相比，糖尿病CKD患者中-344 C>T多态性更为常见（P = 0.02）。TC基因型的糖尿病CKD患者发生该病的风险是无糖尿病的CKD患者的2倍（优势比，2.21；95%置信区间，1.04至4.67）。
我们的研究表明CYP11B2基因-344 C>T多态性可能对糖尿病患者CKD的发生有影响。
