Goswami Chayon, Tanaka Toru, Jogahara Takamichi, Sakai Takafumi, Sakata Ichiro
Area of Regulatory Biology, Division of Life Science, Graduate School of Science and Engineering, Saitama University, Saitama, Japan.
Faculty of Pharmaceutical Sciences, Department of Pharmaceutical and Health Sciences, Josai University, Saitama, Japan.
Biochem Biophys Res Commun. 2015 Jul 3;462(3):263-8. doi: 10.1016/j.bbrc.2015.04.129. Epub 2015 May 6.
Motilin and ghrelin are gastrointestinal hormones that stimulate the migrating motor complex (MMC) of gastrointestinal motility during the fasting state. In this study, we examined the effect of motilin and ghrelin on pepsinogen secretion in anesthetized suncus (house musk shrew, Suncus murinus), a ghrelin- and motilin-producing mammal. By using a gastric lumen-perfusion system, we found that the intravenous administration of carbachol and motilin stimulated pepsinogen secretion, the latter in a dose-dependent manner, whereas ghrelin had no effect. We then investigated the pathways of motilin-induced pepsinogen secretion using acetylcholine receptor antagonists. Treatment with atropine, a muscarinic acetylcholine receptor antagonist, completely inhibited both carbachol and motilin-induced pepsinogen secretion. Motilin-induced pepsinogen secretion was observed in the vagotomized suncus. This is the first report demonstrating that motilin stimulates pepsinogen secretion, and suggest that this effect occurs through a cholinergic pathway in suncus.
胃动素和胃饥饿素是在空腹状态下刺激胃肠动力移行性复合运动（MMC）的胃肠激素。在本研究中，我们研究了胃动素和胃饥饿素对麻醉的臭鼩（家麝鼩，Suncus murinus）胃蛋白酶原分泌的影响，臭鼩是一种能产生胃饥饿素和胃动素的哺乳动物。通过使用胃腔灌注系统，我们发现静脉注射卡巴胆碱和胃动素可刺激胃蛋白酶原分泌，后者呈剂量依赖性，而胃饥饿素无此作用。然后我们使用乙酰胆碱受体拮抗剂研究胃动素诱导胃蛋白酶原分泌的途径。用毒蕈碱型乙酰胆碱受体拮抗剂阿托品处理可完全抑制卡巴胆碱和胃动素诱导的胃蛋白酶原分泌。在迷走神经切断的臭鼩中观察到胃动素诱导的胃蛋白酶原分泌。这是首次证明胃动素刺激胃蛋白酶原分泌的报告，并表明这种作用通过臭鼩的胆碱能途径发生。
