Bouwman Britta A M, de Laat Wouter
Hubrecht Institute - KNAW & University Medical Center Utrecht, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.
Hubrecht Institute - KNAW & University Medical Center Utrecht, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.
FEBS Lett. 2015 Oct 7;589(20 Pt A):2905-13. doi: 10.1016/j.febslet.2015.04.055. Epub 2015 May 7.
Pluripotent stem cells (PSCs) have the ability to self-renew and are capable of generating all embryonic germ layers (Evans and Kaufman, 1981; Thomson et al., 1998). PSCs can be isolated from early embryos or may be induced via overexpression of pluripotency transcription factors in differentiated cells (Takahashi and Yamanaka, 2006). As PSCs hold great promise for regenerative medicine, the mechanisms underlying pluripotency and induction thereof are studied intensively. Pluripotency is characterized by a unique transcriptional program that is in part controlled by an exceptionally plastic regulatory chromatin landscape. In recent years, 3D genome configuration has emerged as an important regulator of transcriptional control and cellular identity (Taddei et al., 2004 [4]; Lanctot et al., 2007 [5]; Gibcus and Dekker, 2013; Misteli, 2009 [7]). Here we provide an overview of recent findings on the 3D genome organization in PSCs and discuss its putative functional role in regulation of the pluripotent state.
多能干细胞（PSCs）具有自我更新能力，能够产生所有胚胎胚层（埃文斯和考夫曼，1981年；汤姆森等人，1998年）。多能干细胞可以从早期胚胎中分离出来，也可以通过在分化细胞中过表达多能性转录因子来诱导产生（高桥和山中伸弥，2006年）。由于多能干细胞在再生医学方面具有巨大潜力，因此对多能性及其诱导机制进行了深入研究。多能性的特征在于独特的转录程序，该程序部分受异常可塑性的调节染色质景观控制。近年来，三维基因组构型已成为转录调控和细胞特性的重要调节因子（塔代伊等人，2004年[4]；兰克托等人，2007年[5]；吉布斯和德克尔，2013年；米斯特利，2009年[7]）。在此，我们概述了多能干细胞中三维基因组组织的最新研究发现，并讨论了其在多能状态调控中的假定功能作用。
