Department of Medical Research, Chia-Yi Christian Hospital, Chia-Yi, Taiwan.
Department of Urology, Chia-Yi Christian Hospital, Chia-Yi, Taiwan.
Virology. 2015 Sep;483:1-12. doi: 10.1016/j.virol.2015.04.009. Epub 2015 May 15.
During polyomavirus infection, the viral DNA adopts histones from host cells and forms minichromosomes as an important part of the viral life cycle. However, the detailed mechanisms of this histone incorporation remain unclear. Here, we profiled the histone posttranslational modifications (PTMs) in BKPyV minichromosomes and in the chromatin of BKPyV host cells. Through Triton-acetic acid-urea (TAU)-PAGE separation followed by nanoflow liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) analysis, we identified different kinds of PTMs on histones from BKPyV minichromosomes and from host cells. We observed not only the common PTMs on histones such as acetylation, methylation, phosphorylation, ubiquitination, and formylation but also several novel PTM sites. Our results also confirmed that the BKPyV minichromosome is hyperacetylated. Our detailed histone PTM profiles for the BKPyV minichromosome provide insights for future exploration of the underlying mechanisms and biological relevance of these histone PTMs.
在多瘤病毒感染过程中，病毒 DNA 采用宿主细胞中的组蛋白，并形成微小染色体，作为病毒生命周期的重要组成部分。然而，这种组蛋白掺入的详细机制仍不清楚。在这里，我们对 BKPyV 微小染色体和 BKPyV 宿主细胞染色质中的组蛋白翻译后修饰 (PTM) 进行了分析。通过 Triton-醋酸-尿素 (TAU) -PAGE 分离，然后进行纳流液相色谱与串联质谱 (LC-MS/MS) 分析，我们鉴定了来自 BKPyV 微小染色体和宿主细胞的组蛋白上不同类型的 PTM。我们不仅观察到组蛋白上常见的 PTM，如乙酰化、甲基化、磷酸化、泛素化和甲酰化，还观察到了几个新的 PTM 位点。我们的结果还证实了 BKPyV 微小染色体的高度乙酰化。我们对 BKPyV 微小染色体的详细组蛋白 PTM 图谱为进一步探索这些组蛋白 PTM 的潜在机制和生物学意义提供了依据。
