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[一株降解二氯喹啉酸的节杆菌属菌株MC-10的筛选、鉴定及特性研究]

[Screening, identification and characterization of a quinclorac-degrading Arthrobacter sp. MC-10].

作者信息

Zhang Shun, Huang Guolian, Xu Jialai, Li Bin, Li Hongguang, Chen Dexin

出版信息

Wei Sheng Wu Xue Bao. 2015 Jan 4;55(1):80-8.

Abstract

OBJECTIVE

We screened and isolated bacterial strains from the perennial administration quinclorac paddy fields to degrade quinclorac.

METHODS

Strain MC-10, which can degrade quinclorac efficiently, was screened by enrichment and selective medium. The strain was identified by morphological, physio-biochemical characteristics and 16S rDNA gene sequence analysis.

RESULTS

MC-10 was identified as Arthrobacter sp. Under the optimal growth conditions with inoculum concentration of 5%, at 28 °C, pH 7 for 7 d, MC-10 degraded more than 90% of quinclorac. MC-10 can effectively degrade quinclorac when quinclorac initial concentration at 1 mg/L--100 mg/L. And the strain MC-10 has a good ability to survive in the soil. More than 70% quinclorac in the soil can be degraded efficiently after 7 days of cultivation.

CONCLUSION

Arthrobacter sp. MC-10 could be a promising microorganism in dealing with quinclorac pollution.

摘要

目的

从长期施用二氯喹啉酸的稻田中筛选并分离能降解二氯喹啉酸的菌株。

方法

通过富集培养和选择性培养基筛选出能高效降解二氯喹啉酸的菌株MC-10,并通过形态学、生理生化特征及16S rDNA基因序列分析对该菌株进行鉴定。

结果

MC-10被鉴定为节杆菌属。在接种浓度5%、28℃、pH 7的最佳生长条件下培养7 d,MC-10对二氯喹啉酸的降解率超过90%。当二氯喹啉酸初始浓度在1 mg/L至100 mg/L时,MC-10能有效降解二氯喹啉酸。且MC-10菌株在土壤中有良好的存活能力,培养7 d后土壤中70%以上的二氯喹啉酸能被高效降解。

结论

节杆菌属MC-10在处理二氯喹啉酸污染方面可能是一种有前景的微生物。

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