[与磁小体形成相关的磁螺菌AMB-1中Mms6在生物矿化中的功能]
[Function of Mms6 related to biomineralization in Magnetospirillum magneticum AMB-1 with magnetosomes formation].
作者信息
Wang Kuan, Ge Xin, Liu Weifeng, Chen Guanjun
出版信息
Wei Sheng Wu Xue Bao. 2015 Feb 4;55(2):187-92.
OBJECTIVE
The function of Mms6 related to biomineralization on the magnetosome formation in Magnetospirillum magneticum AMB-1 was studied.
METHODS
The transcript of mms6 was analyzed under static and aerobic conditions with Real-time RT-PCR. We observed the cell growth and magnetism of the mutation in which mms6 was mutated.
RESULTS
The transcript of mms6 increased with the formation of magnetosomes. Mutation of mms6 caused about 50% decrease of magnetism in AMB-1 under static conditions, however, the cell growth of mutant was similar as to that of the wild type.
CONCLUSION
Gene mms6 is involved in the magnetosome formation of AMB-1.
目的
研究与生物矿化相关的Mms6在嗜磁螺菌AMB-1磁小体形成中的作用。
方法
采用实时逆转录聚合酶链反应（Real-time RT-PCR）分析在静态和好氧条件下mms6的转录情况。观察mms6发生突变的突变体的细胞生长和磁性。
结果
mms6的转录随着磁小体的形成而增加。在静态条件下，mms6的突变导致AMB-1的磁性下降约50%，然而，突变体的细胞生长与野生型相似。
结论
基因mms6参与了AMB-1磁小体的形成。
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