Ozturk Sefa Alperen, Ceylan Cavit, Serel Tekin Ahmet, Doluoglu Omer Gokhan, Soyupek Arap Sedat, Guzel Ahmet, Özorak Alper, Uz Efkan, Savas Hasan Basri, Baspinar Sirin
a Department of Urology Clinic , Suleyman Demirel University, Medical Faculty , Isparta , Turkey .
b Department of Urology Clinic , Yüksek Ihtisas Training and Research Hospital , Ankara , Turkey .
Ren Fail. 2015 Jul;37(6):1044-9. doi: 10.3109/0886022X.2015.1040706. Epub 2015 May 11.
Our objective in this experimental study is to research the effect of the intra-abdominal pressure which rises following pneumoperitoneum and whether Theophylline has a possible protective activity on this situation. In our study, 24 Wistar Albino rats were used. Rats were divided into two groups. The first group was set for only pneumoperitoneum model. The second group was given 15 mg/kg of Theophylline intraperitoneally before setting pneumoperitoneum model. Then urea, creatinine, cystatin-C, tissue and serum total antioxidant capacity, total oxidant capacity and oxidative stress index in two groups were measured and compared with each other. Apoptosis and histopathological conditions in the renal tissues were examined. The differences between the groups were analyzed with the Mann-Whitney U test. Results were considered significant at p < 0.05. No statistically significant difference was determined between tissue and serum averages in two groups in terms of TAS, TOS and OSI values (p > 0.05). The mean value of urea were similar in pneumoperitoneum and pneumoperitoneum + theophylline groups (p = 0.12). The mean cystatin-C value was 2.2 ± 0.3 µg/mL in pneumoperitoneum, 1.74 ± 0.33 µg/mL in pneumoperitoneum + theophylline (p = 0.002). According to our study, lower cystatin-C levels in the group, where Theophylline was given, are suggestive of lower renal injury in this group. However, this opinion is interrogated as there is no difference in terms of tissue and serum TAS, TOS, OSI and urea values between the groups.
本实验研究的目的是探究气腹后腹内压升高的影响，以及茶碱对此情况是否具有潜在的保护作用。在我们的研究中，使用了24只Wistar白化大鼠。大鼠被分为两组。第一组仅设置气腹模型。第二组在设置气腹模型前腹腔注射15mg/kg的茶碱。然后测量两组的尿素、肌酐、胱抑素C、组织和血清总抗氧化能力、总氧化能力和氧化应激指数，并相互比较。检查肾组织中的细胞凋亡和组织病理学情况。组间差异采用Mann-Whitney U检验进行分析。结果在p<0.05时被认为具有统计学意义。在TAS、TOS和OSI值方面，两组的组织和血清平均值之间未确定有统计学显著差异（p>0.05）。气腹组和气腹+茶碱组的尿素平均值相似（p = 0.12）。气腹组的胱抑素C平均值为2.2±0.3μg/mL，气腹+茶碱组为1.74±0.33μg/mL（p = 0.002）。根据我们的研究，给予茶碱的组中较低的胱抑素C水平表明该组肾损伤较低。然而，由于两组在组织和血清TAS、TOS、OSI和尿素值方面没有差异，这一观点受到质疑。
